Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Cyclin Dependent Kinase 1 (CDK1) Polyclonal Antibody | anti-CDK1 antibody
Polyclonal Antibody to Cyclin Dependent Kinase 1 (CDK1)
MGHHHHHHSGSEF- RH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQFM DSSLVKSYLH QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR Y
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
NCBI and Uniprot Product Information
Uniprot Description
CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: CDK family; CDK/CDK1 subfamily; CDK1 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.22; EC 2.7.11.23; Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 10 B5.3|10 36.07 cM
Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytoplasm; cytoskeleton; cytosol; extracellular exosome; membrane; midbody; mitochondrial matrix; mitochondrion; nuclear chromosome, telomeric region; nucleus; spindle microtubule
Molecular Function: ATP binding; chromatin binding; cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; Hsp70 protein binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase II carboxy-terminal domain kinase activity; transferase activity
Biological Process: apoptosis; cell aging; cell cycle; cell differentiation; cell division; cell proliferation; chromosome condensation; DNA repair; Golgi disassembly; histone phosphorylation; microtubule cytoskeleton organization; mitotic cell cycle G2/M transition DNA damage checkpoint; mitotic cell cycle phase transition; negative regulation of apoptosis; organ regeneration; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of cardiac muscle cell proliferation; positive regulation of DNA replication; positive regulation of G2/M transition of mitotic cell cycle; positive regulation of gene expression; positive regulation of mitochondrial ATP synthesis coupled electron transport; positive regulation of mitotic cell cycle; positive regulation of protein import into nucleus, translocation; positive regulation of protein localization to nucleus; protein amino acid phosphorylation; protein complex assembly; protein localization to kinetochore; regulation of gene expression; regulation of meiotic cell cycle; response to ethanol; response to organonitrogen compound
Research Articles on CDK1
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Product Notes
The CDK1 cdk1 (Catalog #AAA2005887) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cyclin Dependent Kinase 1 (CDK1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin Dependent Kinase 1 (CDK1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CDK1 cdk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- RH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQFM DSSLVKSYLH QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR Y. It is sometimes possible for the material contained within the vial of "Cyclin Dependent Kinase 1 (CDK1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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