Rabbit anti-Human, Mouse Cdk1 Polyclonal Antibody | anti-Cdk1 antibody

Cdk1, CT (Cdk1, Cdc2, Cdc2a, Cdkn1, Cyclin-dependent kinase 1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase) (AP)

Cat. Num. AAA6284237

• Gene Names: Cdk1; Cdc2; Cdc2a; p34<CDC2>
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Cdk1; Polyclonal Antibody; CT (Cdk1; Cdc2; Cdc2a; Cdkn1; Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase) (AP); p34 protein kinase; anti-Cdk1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Cdk1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Cdk1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-297 amino acids from the C-terminal region of Mouse Cdk1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Cdk1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Cdk1 antibody detected the Cdk1 protein (arrow).)

Western Blot (WB)

(Mouse Cdk1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Cdk1 antibody detected the Cdk1 protein (arrow).)

Related Product Information for anti-Cdk1 antibody

Cdk1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Product Categories/Family for anti-Cdk1 antibody

Antibodies; Protein Kinases

References

Walker, M.P., et al. J. Endocrinol. 207(2):225-235(2010). Sansregret, L., et al. J. Biol. Chem. 285(43):32834-32843(2010). Van Horn, R.D., et al. J. Biol. Chem. 285(28):21849-21857(2010). Risley, M.D., et al. Dev. Biol. 342(2):146-156(2010). Xu, X.Y., et al. Dev. Dyn. 238(12):3025-3034(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 31542366
• NCBI GeneID: 12534
• NCBI Accession #: NP_031685.2
• NCBI GenBank Nucleotide #: NM_007659.3
• UniProt Accession #: P11440; P70337; Q3TI12
• Molecular Weight: 34,107 Da
• NCBI Official Full Name: cyclin-dependent kinase 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 1
• NCBI Official Symbol: Cdk1
• NCBI Official Synonym Symbols: Cdc2; Cdc2a; p34<CDC2>
• NCBI Protein Information: cyclin-dependent kinase 1; p34 protein kinase; cell division protein kinase 1; cell division cycle 2 homolog A; cell cycle p34 CDC2 kinase protein; cell division cycle control protein 2a; cell division control protein 2 homolog
• UniProt Protein Name: Cyclin-dependent kinase 1
• UniProt Gene Name: Cdk1
• UniProt Synonym Gene Names: Cdc2; Cdc2a; Cdkn1; CDK1

Uniprot Description

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CENPA, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis.