SEPSECS cdna clone

SEPSECS

Cat. Num. AAA200563

• Gene Names: SEPSECS; LP; SLA; PCH2D; SLA/LP
• Synonyms: SEPSECS; LP; PCH2D; SLA; SLA/LP; SEPSECS cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGAACCGCG AGAGCTTCGC GGCGGGAGAG CGGCTGGTGT CGCCGGCTTA CGTGCGGCAG GGCTGTGAGG CCCGCCGCTC GCATGAGCAC CTCATACGGC TGCTTCTGGA GAAGGGCAAG TGTCCAGAGA ATGGCTGGGA TGAAAGTACA CTTGAACTCT TTTTACATGA ACTTGCAATC ATGGACAGCA ACAATTTCTT AGGCAATTGT GGTGTGGGAG AAAGGGAAGG GAGAGTGGCA TCCGCACTGG TTGCTCGTCG TCATTACAGG TTCATTCATG GCATTGGACG ATCCGGTGAT ATTTCTGCTG TGCAACCAAA AGCTGCAGGC TCTAGCCTTT TGAACAAAAT TACCAATTCT TTGGTCCTGG ACATTATAAA GCTGGCTGGT GTCCATACAG TAGCCAACTG CTTTGTAGTT CCTATGGCAA CTGGTATGAG TCTAACTCTG TGTTTCTTAA CATTACGACA CAAAAGACCA AAGGCAAAGT ATATTATATG GCCACGAATA GACCAGAAGT CCTGCTTTAA ATCCATGATC ACTGCAGGTT TTGAGCCTGT GGTGATAGAA AATGTTTTGG AAGGTGACGA GCTGCGTACA GACCTGAAAG CAGTGGAGGC TAAAGTCCAG GAACTTGGGC CTGATTGCAT TCTGTGTATT CATTCTACTA CATCCTGTTT TGCTCCAAGG GTGCCTGATA GATTAGAAGA ACTGGCTGTG ATTTGTGCTA ATTATGACAT TCCACATATA GTTAATAATG CTTATGGAGT GCAGTCTTCA AAGTGTATGC ATCTCATTCA GCAGGGGGCT CGAGTTGGTA GAATAGATGC TTTTGTTCAG AGCTTGGACA AAAATTTTAT GGTTCCAGTA GGTGGTGCTA TAATTGCTGG CTTTAATGAT TCATTCATTC AGGAAATCAG CAAGATGTAT CCAGGAAGAG CTTCAGCTTC ACCTTCTTTA GATGTCCTTA TTACTTTATT GTCACTTGGA TCAAATGGCT ATAAGAAGCT ACTAAAAGAA AGAAAGGAAA TGTTTTCATA TTTGTCCAAC CAAATAAAGA AGTTGTCAGA AGCCTACAAT GAAAGACTGT TGCATACACC TCACAATCCC ATATCTTTAG CTATGACACT TAAAACACTA GATGAACACC GTGACAAAGC TGTCACTCAG CTTGGCTCGA TGCTTTTTAC CAGACAGGTT TCTGGAGCCA GGGTTGTGCC TCTTGGGTCC ATGCAAACTG TGAGTGGCTA TACTTTCAGA GGCTTTATGT CACATACAAA TAATTACCCT TGTGCTTACC TCAATGCTGC ATCAGCCATC GGAATGAAGA TGCAGGATGT GGACCTGTTC ATAAAGAGAC TTGACAGGTG TTTAAAGGCA GTAAGAAAAG AACGAAGTAA AGAGAGTGAT GACAATTATG ACAAAACTGA AGATGTGGAT ATTGAAGAAA TGGCTTTAAA ACTAGATAAT GTACTTCTTG ACACATACCA GGATGCTTCT TCATGA; ; Translation Sequence: MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHI VNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQEISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ LGSMLFTRQV SGARVVPLGS MQTVSGYTFR GFMSHTNNYP CAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERSKESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S
• Sequence Length: 501

• Species: Human
• Chromosome Location: 4p15.2
• OMIM Reference Number: 613009
• cDNA Size: 1506bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for SEPSECS cdna clone

SEPSECS, also known as SLA/LP autoantigen, catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA (sec) to selenocysteinyl-tRNA (sec) using selenophosphate as the selenium donor. Also, this protein is considered a specific marker of autoimmune hepatitis.

Product Categories/Family for SEPSECS cdna clone

Enzymes & Proteases; Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 267844904
• NCBI GeneID: 51091
• NCBI Accession #: NP_058651.3
• NCBI GenBank Nucleotide #: NM_016955.3
• UniProt Accession #: Q9HD40
• NCBI Official Full Name: O-phosphoseryl-tRNA(Sec) selenium transferase
• NCBI Official Synonym Full Names: Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
• NCBI Official Symbol: SEPSECS
• NCBI Official Synonym Symbols: LP; SLA; PCH2D; SLA/LP
• NCBI Protein Information: O-phosphoseryl-tRNA(Sec) selenium transferase
• UniProt Protein Name: O-phosphoseryl-tRNA(Sec) selenium transferase
• Protein Family: O-phosphoseryl-tRNA(Sec) selenium transferase
• UniProt Gene Name: SEPSECS
• UniProt Synonym Gene Names: TRNP48; LP; Sec synthase; SepSecS; SLA
• UniProt Entry Name: SPCS_HUMAN

NCBI Description

The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]

Uniprot Description

SLA/LP: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl- tRNA(Sec) required for selenoprotein biosynthesis. Defects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D). PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures. Belongs to the SepSecS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.9.1.2; Transferase; RNA-binding

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; transferase activity, transferring selenium-containing groups; tRNA binding; pyridoxal phosphate binding

Biological Process: selenocysteine incorporation

Disease: Pontocerebellar Hypoplasia, Type 2d