Western Blot (WB)
(SEPSECS Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SEPSECS Antibody detected the SEPSECS protein (arrow).)
Rabbit anti-Human SEPSECS Polyclonal Antibody | anti-SEPSECS antibody
SEPSECS, CT (SEPSECS, TRNP48, O-phosphoseryl-tRNA(Sec) selenium transferase, Liver-pancreas antigen, SLA-p35, SLA/LP autoantigen, Selenocysteine synthase, Selenocysteinyl-tRNA(Sec) synthase, Sep-tRNA:Sec-tRNA synthase, Soluble liver antigen, UGA suppresso
Gene Names
SEPSECS; LP; SLA; PCH2D; SLA/LP
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SEPSECS; Polyclonal Antibody; CT (SEPSECS; TRNP48; O-phosphoseryl-tRNA(Sec) selenium transferase; Liver-pancreas antigen; SLA-p35; SLA/LP autoantigen; Selenocysteine synthase; Selenocysteinyl-tRNA(Sec) synthase; Sep-tRNA:Sec-tRNA synthase; Soluble liver antigen; UGA suppresso; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein; anti-SEPSECS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-SEPSECS antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
SEPSECS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-474 amino acids from the C-terminal region of human SEPSECS.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(SEPSECS Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SEPSECS Antibody detected the SEPSECS protein (arrow).)
Western Blot (WB)
(SEPSECS Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SEPSECS Antibody detected the SEPSECS protein (arrow).)
Related Product Information for anti-SEPSECS antibody
The 21st amino acid, selenocysteine (sec), is distinct from other amino acids because it lacks its own tRNA synthetase and is the only amino acid synthesized on its cognate tRNA. Synthesis of sec begins with acylation of tRNA(sec) (TRSP; MIM 165060) by seryl-tRNA synthetase (SARS; MIM 607529) to give ser-tRNA(sec), which is subsequently phosphorylated by O-phosphoseryl-tRNA kinase (PSTK; MIM 611310) to give O-phosphoseryl-tRNA(sec). SEPSECS catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor (Palioura et al., 2009 [PubMed 19608919]).
Product Categories/Family for anti-SEPSECS antibody
References
Agamy, O., et al. Am. J. Hum. Genet. 87(4):538-544(2010). Hart, K., et al. Lung Cancer (2010). In press: Volkmann, M., et al. J. Autoimmun. 34(1):59-65(2010). Palioura, S., et al. Science 325(5938):321-325(2009). Xu, X.M., et al. PLoS Biol. 5 (1), E4 (2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,411 Da
NCBI Official Full Name
O-phosphoseryl-tRNA(Sec) selenium transferase
NCBI Official Synonym Full Names
Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
NCBI Official Symbol
SEPSECS
NCBI Official Synonym Symbols
LP; SLA; PCH2D; SLA/LP
NCBI Protein Information
O-phosphoseryl-tRNA(Sec) selenium transferase; O-phosphoseryl-tRNA(Sec) selenium transferase; SLA-p35; SLA/LP autoantigen; UGA suppressor tRNA-associated protein; liver-pancreas antigen; soluble liver antigen; soluble liver antigen/liver pancreas antigen;
UniProt Protein Name
O-phosphoseryl-tRNA(Sec) selenium transferase
Protein Family
UniProt Gene Name
SEPSECS
UniProt Synonym Gene Names
TRNP48; LP; Sec synthase; SepSecS; SLA
UniProt Entry Name
SPCS_HUMAN
NCBI Description
The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]
Uniprot Description
SLA/LP: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl- tRNA(Sec) required for selenoprotein biosynthesis. Defects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D). PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures. Belongs to the SepSecS family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.9.1.2; Transferase; RNA-binding
Chromosomal Location of Human Ortholog: 4p15.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; transferase activity, transferring selenium-containing groups; tRNA binding; pyridoxal phosphate binding
Biological Process: selenocysteine incorporation
Disease: Pontocerebellar Hypoplasia, Type 2d
Research Articles on SEPSECS
Similar Products
Product Notes
The SEPSECS sepsecs (Catalog #AAA6346119) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEPSECS, CT (SEPSECS, TRNP48, O-phosphoseryl-tRNA(Sec) selenium transferase, Liver-pancreas antigen, SLA-p35, SLA/LP autoantigen, Selenocysteine synthase, Selenocysteinyl-tRNA(Sec) synthase, Sep-tRNA:Sec-tRNA synthase, Soluble liver antigen, UGA suppresso reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SEPSECS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SEPSECS sepsecs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEPSECS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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