Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Profilin 3 (PFN3) Polyclonal Antibody | anti-PFN3 antibody
Polyclonal Antibody to Profilin 3 (PFN3)
Gene Names
Pfn3; 1700012P12Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Profilin 3 (PFN3); Polyclonal Antibody; Polyclonal Antibody to Profilin 3 (PFN3); anti-PFN3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PFN3. It has been selected for its ability to recognize PFN3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-MSDWKGYISA VLRDQRIDDV AIVGHSDNRC VWASRPGGLL AAISPQEVGV LTGPDRHTFL QTGLSVAGRR CCVIRDYLLA EGDGVLDART KGLDGRAICV GHTPRALLVL MGRRGVHGGI LNKTVHDLIG GL
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-MSDWKGYISA VLRDQRIDDV AIVGHSDNRC VWASRPGGLL AAISPQEVGV LTGPDRHTFL QTGLSVAGRR CCVIRDYLLA EGDGVLDART KGLDGRAICV GHTPRALLVL MGRRGVHGGI LNKTVHDLIG GL
Sequence Length
145
Applicable Applications for anti-PFN3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant PFN3 (Met1~Leu132) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2075958
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Skeletal muscle Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Skeletal muscle Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,697 Da
NCBI Official Full Name
profilin-3
NCBI Official Synonym Full Names
profilin 3
NCBI Official Symbol
Pfn3
NCBI Official Synonym Symbols
1700012P12Rik
NCBI Protein Information
profilin-3
UniProt Protein Name
Profilin-3
Protein Family
UniProt Gene Name
Pfn3
Uniprot Description
PFN3: Binds to actin and affects the structure of the cytoskeleton. May be involved in spermatogenesis. Belongs to the profilin family.
Protein type: Actin-binding; Cytoskeletal
Chromosomal Location of Human Ortholog: 13 B1|13 30.06 cM
Cellular Component: cytoplasm; cytoskeleton; nucleus
Molecular Function: actin binding; lipid binding; protein binding
Biological Process: actin cytoskeleton organization; positive regulation of actin filament bundle assembly; regulation of actin filament polymerization
Research Articles on PFN3
Similar Products
Product Notes
The PFN3 pfn3 (Catalog #AAA2003951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Profilin 3 (PFN3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Profilin 3 (PFN3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PFN3 pfn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -MSDWKGYIS A VLRDQRIDDV AIVGHSDNRC VWASRPGGLL AAISPQEVGV LTGPDRHTFL QTGLSVAGRR CCVIRDYLLA EGDGVLDART KGLDGRAICV GHTPRALLVL MGRRGVHGGI LNKTVHDLIG GL. It is sometimes possible for the material contained within the vial of "Profilin 3 (PFN3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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