Rabbit Complement Component 4b (C4b) Antibody | anti-C4b antibody

Polyclonal Antibody to Complement Component 4b (C4b)

Cat. Num. AAA2003827

• Gene Names: C4b; C4; Ss
• Reactivity: Mus musculus (Mouse)
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Complement Component 4b (C4b); Polyclonal Antibody to Complement Component 4b (C4b); anti-C4b antibody

• Host: Rabbit
• Reactivity: Mus musculus (Mouse)
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-C4b antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western blotting: 0.5-3ug/mL; Immunohistochemistry: 5-30ug/mL; Immunocytochemistry: 5-30ug/mL; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Rat
• Immunogen: Recombinant C4B (Gln126~Ser366) expressed in E.coli (MBS2010348).
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Recombinant C4b, Mouse.)

Western Blot (WB)

(Western BlotSample:Lane1: Mouse PlasmaLane2: Rat SerumLane3: Rat PlasmaPrimary Ab: 1ug/ml Rabbit Anti-Mouse C4a AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Small intestine Tissue;Primary Ab: 30ug/ml Rabbit Anti-Mouse C4a AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

NCBI and Uniprot Product Information

• NCBI GI #: 157951698
• NCBI GeneID: 12268
• NCBI Accession #: NP_033910.2
• NCBI GenBank Nucleotide #: NM_009780.2
• UniProt Accession #: P01029; O70346; Q31201; Q3TYY1; Q3TZC9; Q61372; Q61859; Q62353; Q6NWV8; E9QKK7
• NCBI Official Full Name: complement C4-B
• NCBI Official Synonym Full Names: complement component 4B (Chido blood group)
• NCBI Official Symbol: C4b
• NCBI Official Synonym Symbols: C4; Ss
• NCBI Protein Information: complement C4-B
• UniProt Protein Name: Complement C4-B
• Protein Family: Complement C4
• UniProt Gene Name: C4b
• UniProt Synonym Gene Names: C4

Uniprot Description

C4B: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4B are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE. Defects in C4B are the cause of complement component 4B deficiency (C4BD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17 B1|17 18.29 cM

Cellular Component: axon; cell soma; dendrite; extracellular region; extracellular space; synapse

Molecular Function: carbohydrate binding; complement binding; complement component C1q binding

Biological Process: complement activation; immunoglobulin mediated immune response