Rabbit anti-Human Complement C4-beta chain Polyclonal Antibody | anti-C4b antibody

Anti-Complement C4-beta chain antibody

Cat. Num. AAA355172

• Gene Names: C4b; C4; Ss
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified
• Synonyms: Complement C4-beta chain; Polyclonal Antibody; Anti-Complement C4-beta chain antibody; Polyclonal Anti- Complement C4-beta chain; Polyclonal Anti- Complement C4-b chain; anti-C4b antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified
• Form/Format: Liquid; In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Concentration: 2 mg/ml (varies by lot)

• Applicable Applications for anti-C4b antibody: Western Blot (WB) - Other applications have not been tested.
• Application Notes: Recommended Dilution: Western Blot (1:1000) - The optimal dilutions should be determined by end user.
• Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the region of human ApoE.
• Preparation and Storage: Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Western Blot (WB)

Related Product Information for anti-C4b antibody

Complement C4 encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.

References

1. Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665-76. 2. Singh PP, Singh M, Mastana SS (2002). "Genetic variation of apolipoproteins in North Indians". Hum. Biol. 74 (5): 673-82.

NCBI and Uniprot Product Information

• NCBI GI #: 157951698
• NCBI GeneID: 12268
• NCBI Accession #: NP_033910.2
• NCBI GenBank Nucleotide #: NM_009780.2
• UniProt Accession #: P01029; O70346; Q31201; Q3TYY1; Q3TZC9; Q61372; Q61859; Q62353; Q6NWV8; E9QKK7
• NCBI Official Full Name: complement C4-B
• NCBI Official Synonym Full Names: complement component 4B (Chido blood group)
• NCBI Official Symbol: C4b
• NCBI Official Synonym Symbols: C4; Ss
• NCBI Protein Information: complement C4-B; complement component 4 (within H-2S); complement component 4B (Childo blood group)
• UniProt Protein Name: Complement C4-B
• Protein Family: Complement C4
• UniProt Gene Name: C4b
• UniProt Synonym Gene Names: C4
• UniProt Entry Name: CO4B_MOUSE

Uniprot Description

Function: Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens

By similarity.

Subunit structure: Circulates in blood as a disulfide-linked trimer of an alpha, beta and gamma chain.

Subcellular location: Secreted.

Post-translational modification: Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.

Miscellaneous: C4 is a major histocompatibility complex class-III protein.

Sequence similarities: Contains 1 anaphylatoxin-like domain.Contains 1 NTR domain.