Western Blot (WB)
(Western Blot: Sample: Recombinant NEP, Rat.)
Rabbit anti-Rat Neprilysin (NEP) Polyclonal Antibody | anti-NEP antibody
Polyclonal Antibody to Neprilysin (NEP)
MGHHHHHHSGSEF-SVLVLLLTI IAVTMIALYA TYDDGICKSS DCIKSAARLI QNMDASAEPC TDFFKYACGG WLKRNVIPET SSRYSNFDIL RDELEVILKD VLQEPKTEDI VAVQKAKTLY RSCINESAID SRGGQPLLTL LPDI
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant NEP, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NEP, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue.)
NCBI and Uniprot Product Information
NCBI Description
zinc-containing metallo-enzyme; involved in degradation of cardiac peptides [RGD, Feb 2006]
Uniprot Description
CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.
Protein type: EC 3.4.24.11; Membrane protein, integral; Protease
Chromosomal Location of Human Ortholog: 2q31
Cellular Component: axon; brush border; cytoplasm; dendrite; focal adhesion; integral component of membrane; membrane; plasma membrane; synapse; synaptic vesicle
Molecular Function: endopeptidase activity; exopeptidase activity; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; peptide binding; zinc ion binding
Biological Process: beta-amyloid metabolic process; creatinine metabolic process; kidney development; peptide metabolic process; proteolysis; sensory perception of pain
Research Articles on NEP
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Product Notes
The NEP mme (Catalog #AAA2003782) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Neprilysin (NEP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neprilysin (NEP) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NEP mme for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-SVLVLL LTI IAVTMIALYA TYDDGICKSS DCIKSAARLI QNMDASAEPC TDFFKYACGG WLKRNVIPET SSRYSNFDIL RDELEVILKD VLQEPKTEDI VAVQKAKTLY RSCINESAID SRGGQPLLTL LPDI. It is sometimes possible for the material contained within the vial of "Neprilysin (NEP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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