Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Slit Homolog 2 (Slit2) Polyclonal Antibody | anti-Slit2 antibody
Polyclonal Antibody to Slit Homolog 2 (Slit2)
MGHHHHHHSGS-NN LYCDCHLAWL SDWLRQRPRV GLYTQCMGPS HLRGHNVAEV QKREFVCSDE EEGHQSFMAP SCSVLHCPIA CTCSNNIVDC RGKGLTEIPT NLPETITEIR LEQNSIRVIP PGAFSPYKKL RRLDLSNNQI SELAPDAFQG LRSLNSLVLY GNKITELPKS LFEG
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI Description
acts as a ligand for glypican-1, a heparan sulfate proteoglycan; may play a role in neurogenesis and midline development [RGD, Feb 2006]
Uniprot Description
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. Seems to be involved in regulating leukocyte migration ().
Research Articles on Slit2
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Product Notes
The Slit2 slit2 (Catalog #AAA2002144) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Slit Homolog 2 (Slit2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Slit Homolog 2 (Slit2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the Slit2 slit2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S-NN LYCDCHLAWL SDWLRQRPRV GLYTQCMGPS HLRGHNVAEV QKREFVCSDE EEGHQSFMAP SCSVLHCPIA CTCSNNIVDC RGKGLTEIPT NLPETITEIR LEQNSIRVIP PGAFSPYKKL RRLDLSNNQI SELAPDAFQG LRSLNSLVLY GNKITELPKS LFEG. It is sometimes possible for the material contained within the vial of "Slit Homolog 2 (Slit2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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