Typical Testing Data/Standard Curve (for reference only)
Fibroblast Growth Factor Receptor Substrate 3 (FRS3) CLIA Kit | FRS3 clia kit
CLIA Kit for Fibroblast Growth Factor Receptor Substrate 3 (FRS3)
Gene Names
FRS3; SNT2; FRS2B; SNT-2; FRS2beta; FRS2-beta
Synonyms
Fibroblast Growth Factor Receptor Substrate 3 (FRS3); CLIA Kit for Fibroblast Growth Factor Receptor Substrate 3 (FRS3); FRS3 clia kit
Specificity
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor Receptor Substrate 3 (FRS3)
No significant cross-reactivity or interference between Fibroblast Growth Factor Receptor Substrate 3 (FRS3) and analogues was observed.
No significant cross-reactivity or interference between Fibroblast Growth Factor Receptor Substrate 3 (FRS3) and analogues was observed.
Sequence Length
492
Assay Type
Sandwich
Samples
Tissue homogenates and other biological fluids
Detection Range
0.014-10ng/mL The standard curve concentrations used for the ELISA's were 10ng/mL, 3.33ng/mL, 1.11ng/mL, 0.37ng/mL, 0.123ng/mL, 0.041ng/mL, 0.014ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.059ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor Receptor Substrate 3 (FRS3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor Receptor Substrate 3 (FRS3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor Receptor Substrate 3 (FRS3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Organism Species
Rattus norvegicus (Rat)
Assay Length
4.5 hours
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FRS3 clia kit
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor Receptor Substrate 3 (FRS3) level in the sample or standard.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,462 Da
NCBI Official Full Name
fibroblast growth factor receptor substrate 3
NCBI Official Synonym Full Names
fibroblast growth factor receptor substrate 3
NCBI Official Symbol
FRS3
NCBI Official Synonym Symbols
SNT2; FRS2B; SNT-2; FRS2beta; FRS2-beta
NCBI Protein Information
fibroblast growth factor receptor substrate 3; FGFR substrate 3; FGFR-signaling adaptor SNT2; suc1-associated neurotrophic factor target 2 (FGFR signalling adaptor)
UniProt Protein Name
Fibroblast growth factor receptor substrate 3
Protein Family
UniProt Gene Name
FRS3
UniProt Synonym Gene Names
FGFR substrate 3; SNT-2
UniProt Entry Name
FRS3_HUMAN
NCBI Description
This gene encodes a substrate for the fibroblast growth factor receptor. The encoded protein is found in the peripheral plasma membrane and links fibroblast growth factor receptor stimulation to activators of Ras. The encoded protein down-regulates extracellular regulated kinase 2 through direct binding. [provided by RefSeq, Jul 2013]
Uniprot Description
FRS3: Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: plasma membrane
Molecular Function: protein binding; insulin receptor binding; fibroblast growth factor receptor binding
Biological Process: fibroblast growth factor receptor signaling pathway; signal transduction
Research Articles on FRS3
Similar Products
Product Notes
The FRS3 frs3 (Catalog #AAA2001023) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor Receptor Substrate 3 (FRS3), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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