Rabbit Bassoon Polyclonal Antibody | anti-Bsn antibody

Bassoon

Cat. Num. AAA194510

• Applications: Immunoblot, Western Blot, Immunohistochemistry, Immunofluorescence
• Synonyms: Bassoon; Polyclonal Antibody; Anti-Bassoon; anti-Bsn antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody recognizes human, mouse, and rat Bassoon. Multiple isoforms are detected.
• Sequence Length: 3942

• Applicable Applications for anti-Bsn antibody: Immunoblot (IB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Immunoblotting: Use at 1ug/ml.; Immunohistochemistry: Use at 1-10ug/ml.; Immunofluorescence: Use at 1-10ug/ml.
• Antigen: Fusion protein corresponding to aa786-1041 (N-terminal) of human Bassoon (accession no. NM_003458.3).
• Positive Control: Rat brain tissue lysate
• Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freeze-thaw cycles.

Testing Data

Testing Data

Related Product Information for anti-Bsn antibody

Bassoon, a 420kDa protein, is localized at presynaptic nerve terminals and appears to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The C-terminal regions of the polyglutamine domains are of interest since abnormal amplification of this region in some human proteins, such as Huntingtin, can cause late-onset neuro- degeneration.

Product Categories/Family for anti-Bsn antibody

Neuroscience and Signal Transduction Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 3413810
• UniProt Accession #: O88737; Q6ZQB5; E9QMZ3
• Molecular Weight: 412,264 Da
• NCBI Official Full Name: Bassoon
• UniProt Protein Name: Protein bassoon
• Protein Family: Protein bassoon
• UniProt Gene Name: Bsn
• UniProt Synonym Gene Names: Kiaa0434
• UniProt Entry Name: BSN_MOUSE

Uniprot Description

BSN: involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses. Interacts with CAST1, RIMS1 and UNC13A. Part of a complex consisting of ERC2, RIMS1 and BSN. Localized to the active zone of presynaptic density. Mice lacking functional BSN showed a reduced excitability attributed to inactivation of a fraction of brain glutamatergic synapses. At these synapses, vesicles are clustered and docked in normal numbers, but are unable to fuse. In retina, mutants lacking functional BSN showed normal retinal anatomy, but synapses lacked anchoring of the photoreceptor ribbon to the presynaptic active zone resulting in impaired photoreceptor synaptic transmission. Two alternatively spliced isoforms have been reported.

Protein type: Cytoskeletal

Cellular Component: axon; cell junction; cell soma; cell surface; cytoplasm; cytoskeleton; dendrite; excitatory synapse; extrinsic to membrane; integral to membrane; neuron projection; postsynaptic density; presynaptic active zone; synapse; trans-Golgi network

Molecular Function: metal ion binding; protein binding

Biological Process: synaptogenesis