Mouse CD81 Monoclonal Antibody | anti-CD81 antibody

Anti-Hu CD81 Pacific Blue

• Gene Names: CD81; S5.7; CVID6; TAPA1; TSPAN28
• Reactivity: Rabbit, Cat, Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD81; Monoclonal Antibody; Anti-Hu CD81 Pacific Blue; S5.7; CVID6; TAPA1; TSPAN28; CD81 molecule; anti-CD81 antibody

• Host: Mouse
• Reactivity: Rabbit, Cat, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: M38
• Specificity: The antibody M38 reacts with an extracellular epitope of CD81, a 25kDa member of the tetraspanin family, expressed on majority of cells.
• Purity/Purification: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Pacific Blue. Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD81 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Immunogen: MOLT-4 (human T-ALL cell line)
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Related Product Information for anti-CD81 antibody

CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.

References

*Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1;151(11):6470-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/8245480ordina,*Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1;155(3):1229-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/7636191ordina,*Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7;273(32):20121-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9685355ordina,*Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar;66(3):1394-401., URL: http://www.ncbi.nlm.nih.gov/pubmed/1738199ordinal,*Stehlíková O, Chovancová J, Tichý B, Krej?í M, Brychtová Y, Panovská A, Francová Skuhrová H, Bur?ková K, Borský M, Loja T, Mayer J, Pospíšilová S, Doubek M: Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8-color flow cytometry protocol in routine hematological practice. Int J Lab Hematol. 2013 Sep 13. doi: 10.1111/ijlh.12149., URL: http://www.ncbi.nlm.nih.gov/pubmed/24028768,*Fritzsching B, Schwer B, Kartenbeck J, Pedal A, Horejsi V, Ott M: Release and intercellular transfer of cell surface CD81 via microparticles. J Immunol. 2002 Nov 15;169(10):5531-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=schwer+b+pedal+a

NCBI and Uniprot Product Information

• NCBI GI #: 38503376
• NCBI GeneID: 975
• UniProt Accession #: P60033; P18582; Q5U0J6
• Molecular Weight: 25,809 Da
• NCBI Official Full Name: CD81 antigen
• NCBI Official Synonym Full Names: CD81 molecule
• NCBI Official Symbol: CD81
• NCBI Official Synonym Symbols: S5.7; CVID6; TAPA1; TSPAN28
• NCBI Protein Information: CD81 antigen
• UniProt Protein Name: CD81 antigen
• Protein Family: CD81 antigen
• UniProt Gene Name: CD81
• UniProt Synonym Gene Names: TAPA1; TSPAN28; Tspan-28

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.