Mouse CD64 Monoclonal Antibody | anti-CD64 antibody

Anti-Hu CD64 PE-Cyanine7

Cat. Num. AAA1800010

• Gene Names: FCGR1A; CD64; FCRI; CD64A; IGFR1
• Reactivity: Human, Non-human primates
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with tandem dye PE-Cyanine7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD64; Monoclonal Antibody; Anti-Hu CD64 PE-Cyanine7; Fc fragment of IgG receptor Ia; FcRI; IGFR1; FcgR1A; anti-CD64 antibody

• Host: Mouse
• Reactivity: Human, Non-human primates
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 10.1
• Specificity: The mouse monoclonal antibody 10.1 recognizes an extracellular epitope on CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes.
• Purity/Purification: The purified antibody is conjugated with tandem dye PE-Cyanine7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
• Sequence Length: 374

• Applicable Applications for anti-CD64 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: Rheumatoid synovial fluid cells and fibronectin purified human monocytes
• Conjugation: PE-Cyanine7
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of CD64 in human peripheral blood with anti-CD64 (10.1) PE-Cy7.)

Related Product Information for anti-CD64 antibody

CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 50403717
• NCBI GeneID: 2209
• UniProt Accession #: P12314
• NCBI Official Full Name: High affinity immunoglobulin gamma Fc receptor I
• NCBI Official Synonym Full Names: Fc fragment of IgG receptor Ia
• NCBI Official Symbol: FCGR1A
• NCBI Official Synonym Symbols: CD64; FCRI; CD64A; IGFR1
• NCBI Protein Information: high affinity immunoglobulin gamma Fc receptor I
• UniProt Protein Name: High affinity immunoglobulin gamma Fc receptor I
• UniProt Gene Name: FCGR1A
• UniProt Synonym Gene Names: FCG1; FCGR1; IGFR1; IgG Fc receptor I; FcRI; FcgammaRIa
• UniProt Entry Name: FCGR1_HUMAN

NCBI Description

This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

FCGR1A: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Belongs to the immunoglobulin superfamily. FCGR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21.2-q21.3

Cellular Component: early endosome membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; IgG binding; receptor signaling protein activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; signal transduction; phagocytosis, engulfment