Mouse CD64 Monoclonal Antibody | anti-CD64 antibody

Anti-Hu CD64 Pacific Blue

• Gene Names: FCGR1A; CD64; FCRI; CD64A; IGFR1
• Reactivity: Human, Non-human primates
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD64; Monoclonal Antibody; Anti-Hu CD64 Pacific Blue; FcRI; IGFR1; FcgR1A; Fc fragment of IgG receptor Ia; anti-CD64 antibody

• Host: Mouse
• Reactivity: Human, Non-human primates
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: 10.1
• Specificity: The mouse monoclonal antibody 10.1 recognizes an extracellular epitope on CD64/FcgammaRI, a 72kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes.
• Purity/Purification: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Pacific Blue. Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD64 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Immunogen: Rheumatoid synovial fluid cells and fibronectin purified human monocytes
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of CD64 in human peripheral blood with anti-CD64 (10.1) Pacific Blue™.)

Related Product Information for anti-CD64 antibody

CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector Functional Application (FUNC)tions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if Functional Application (FUNC)tionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.

References

*Devaraj S, Du Clos TW, Jialal I: Binding and internalization of C-reactive protein by Fcgamma receptors on human aortic endothelial cells mediates biological effects. Arterioscler Thromb Vasc Biol. 2005 Jul;25(7):1359-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/15860734,*Devaraj S, Davis B, Simon SI, Jialal I: CRP promotes monocyte-endothelial cell adhesion via Fcgamma receptors in human aortic endothelial cells under static and shear flow conditions. Am J Physiol Heart Circ Physiol. 2006 Sep;291(3):H1170-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/16603696,*Jayaram Y, Buckle AM, Hogg N: The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon-gamma. Clin Exp Immunol. 1989 Mar;75(3):414-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/2495203,*Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382,*Hashimoto S, Yamada M, Motoyoshi K, Akagawa KS: Enhancement of macrophage colony-stimulating factor-induced growth and differentiation of human monocytes by interleukin-10. Blood. 1997 Jan 1;89(1):315-21., URL: https://www.ncbi.nlm.nih.gov/pubmed/8978307,*Fadlon E, Vordermeier S, Pearson TC, Mire-Sluis AR, Dumonde DC, Phillips J, Fishlock K, Brown KA: Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64. Blood. 1998 Jan 1;91(1):266-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/9414294,*Brichard B, Varis I, Latinne D, Deneys V, de Bruyere M, Leveugle P, Cornu G: Intracellular cytokine profile of cord and adult blood monocytes. Bone Marrow Transplant. 2001 May;27(10):1081-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/11438825,*Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int Immunol. 2001 Dec;13(12):1571-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/11717198,*Beekman JM, Bakema JE, van der Linden J, Tops B, Hinten M, van Vugt M, van de Winkel JG, Leusen JH: Modulation of FcgammaRI (CD64) ligand binding by blocking peptides of periplakin. J Biol Chem. 2004 Aug 6;279(32):33875-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/15161926,*Roura-Mir C, Wang L, Cheng TY, Matsunaga I, Dascher CC, Peng SL, Fenton MJ, Kirschning C, Moody DB: Mycobacterium tuberculosis regulates CD1 antigen presentation pathways through TLR-2. J Immunol. 2005 Aug 1;175(3):1758-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/16034117,*Dougherty GJ, Selvendran Y, Murdoch S, Palmer DG, Hogg N: The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1. Eur J Immunol. 1987 Oct;17(10):1453-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/3500057,*Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2019 Nov 8. doi: 10.1002/cyto.b.21853., URL: https://www.ncbi.nlm.nih.gov/pubmed/31705743,*Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94(1):23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353

NCBI and Uniprot Product Information

• NCBI GI #: 50403717
• NCBI GeneID: 2209
• UniProt Accession #: P12314; P12315; Q5QNW7; Q92495; Q92663
• Molecular Weight: 374
• NCBI Official Full Name: High affinity immunoglobulin gamma Fc receptor I
• NCBI Official Synonym Full Names: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• NCBI Official Symbol: FCGR1A
• NCBI Official Synonym Symbols: CD64; FCRI; CD64A; IGFR1
• NCBI Protein Information: high affinity immunoglobulin gamma Fc receptor I; fcgammaRIa; Fc-gamma RI; fc-gamma RIA; Fc gamma receptor; IgG Fc receptor I; Fc-gamma receptor I A1; Fc fragment of IgG, high affinity Ia, receptor for (CD64)
• UniProt Protein Name: High affinity immunoglobulin gamma Fc receptor I
• UniProt Gene Name: FCGR1A
• UniProt Synonym Gene Names: FCG1; FCGR1; IGFR1; IgG Fc receptor I; FcRI; FcgammaRIa
• UniProt Entry Name: FCGR1_HUMAN

NCBI Description

This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

FCGR1A: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Belongs to the immunoglobulin superfamily. FCGR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21.2-q21.3

Cellular Component: early endosome membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; IgG binding; receptor signaling protein activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; signal transduction; phagocytosis, engulfment