Rabbit CD2 Polyclonal Antibody | anti-CD2 antibody

Anti-CD2 Antibody

Cat. Num. AAA178429

• Gene Names: Cd2; Ly37; LFA-2; Ly-37
• Reactivity: Mouse; No cross reactivity with other proteins.
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: CD2; Polyclonal Antibody; Anti-CD2 Antibody; CD2 antigen; CD2 molecule; LFA2; LFA-2; Rosette receptor; SRBC; T cell surface antigen CD2; T11; Tcell surface antigen CD2; T-cell surface antigen CD2; T-cell surface antigen T11; P08920 ; anti-CD2 antibody

• Host: Rabbit
• Reactivity: Mouse; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 344

• Applicable Applications for anti-CD2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot: 0.1-0.5mug/ml; Tested Species: Mouse; Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Mouse; Tested Species:In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.
• Immunogen: E Coli-derived mouse CD2 recombinant protein (Position: R23-S203). Mouse CD2 shares 49.7% and 73.3% amino acid (aa) sequence identity with human and rat CD2, respectively.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of CD2 expression in mouse cardiac muscle extract (lane 1) and HEPA whole cell lysates (lane 2). CD2 at 58KD was detected using rabbit anti- CD2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(CD2 was detected in paraffin-embedded sections of mouse lymphaden tissues using rabbit anti- CD2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-CD2 antibody

Rabbit IgG polyclonal antibody for T-cell surface antigen CD2 (CD2) detection.
Background: CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portion. The localization of CD2 to 1p13 is established by in situ hybridization. CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR is identified within the 3-prime flanking region of the human CD2 gene.

References

1. Brown, M. H., Gorman, P. A., Sewell, W. A., Spurr, N. K., Sheer, D., Crumpton, M. J. The gene coding for the human T-lymphocyte CD2 antigen is located on chromosome 1p. Hum. Genet. 76: 191-195, 1987.
2. Clayton, L. K., Ramachandran, H., Pravtcheva, D., Chen, Y.-F., Diamond, D. J., Ruddle, F. H., Reinherz, E. L. The gene for T11 (CD2) maps to chromosome 1 in humans and to chromosome 3 in mice. J. Immun. 140: 3617-3621, 1988.
3. Kingsmore, S. F., Watson, M. L., Moseley, W. S., Seldin, M. F. Physical linkage of genes encoding the lymphocyte adhesion molecules CD2 and its ligand LFA-3. Immunogenetics 30: 123-125, 1989.

NCBI and Uniprot Product Information

• NCBI GI #: 7304949
• NCBI GeneID: 12481
• NCBI Accession #: NP_038514.1
• NCBI GenBank Nucleotide #: NM_013486.2
• UniProt Accession #: P08920; Q61394
• Molecular Weight: 38,415 Da
• NCBI Official Full Name: T-cell surface antigen CD2
• NCBI Official Synonym Full Names: CD2 antigen
• NCBI Official Symbol: Cd2
• NCBI Official Synonym Symbols: Ly37; LFA-2; Ly-37
• NCBI Protein Information: T-cell surface antigen CD2
• UniProt Protein Name: T-cell surface antigen CD2
• Protein Family: CD2
• UniProt Gene Name: Cd2
• UniProt Synonym Gene Names: Ly-37; Ly-37
• UniProt Entry Name: CD2_MOUSE

Uniprot Description

CD2: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.

Protein type: Cell surface; Apoptosis; Membrane protein, integral

Cellular Component: anchored to plasma membrane; cell surface; external side of plasma membrane; extracellular region; intercellular junction; internal side of plasma membrane; lipid raft; protein complex

Molecular Function: antigen binding; glycoprotein binding; protein binding; protein homodimerization activity; protein kinase binding; protein self-association; receptor binding; receptor tyrosine kinase binding

Biological Process: cell-cell adhesion; heterotypic cell-cell adhesion; positive regulation of tumor necrosis factor production; T cell activation