Western Blot (WB)
(Anti- PSA Picoband antibody, MBS178309, Western blottingAll lanes: Anti PSA (MBS178309) at 0.5ug/mlWB: Recombinant Human PSA Protein 0.5ngPredicted bind size: 25KDObserved bind size: 25KD )
anti-Human, Rat PSA Polyclonal Antibody | anti-PSA antibody
Anti-PSA Antibody
WB: The detection limit for PSA is approximately 0.2ng/lane under reducing conditions.
Tested Species: In-house tested species with positives results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Western Blot (WB)
(Anti- PSA Picoband antibody, MBS178309, Western blottingAll lanes: Anti PSA (MBS178309) at 0.5ug/mlWB: Recombinant Human PSA Protein 0.5ngPredicted bind size: 25KDObserved bind size: 25KD )
Western Blot (WB)
(Anti- PSA Picoband antibody, MBS178309, Western blottingAll lanes: Anti PSA (MBS178309) at 0.5ug/mlWB: Recombinant Human PSA Protein 0.5ngPredicted bind size: 25KDObserved bind size: 25KD )
Background: Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme encoded in humans by the KLK3 gene. KLK3 is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. This gene is mapped to 19q13.33. KLK3 is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. KLK3 is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm. It is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia.
NCBI and Uniprot Product Information
NCBI Description
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.
Protein type: EC 3.4.21.77; Protease; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: extracellular region; extracellular space; nucleus; protein complex
Molecular Function: endopeptidase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; protein binding; serine-type endopeptidase activity; serine-type peptidase activity
Biological Process: antibacterial peptide production; cellular protein metabolic process; negative regulation of angiogenesis; proteolysis
Research Articles on PSA
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Product Notes
The PSA klk3 (Catalog #AAA178309) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSA Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PSA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml; Tested Species: Human, Rat WB: The detection limit for PSA is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positives results. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the PSA klk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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