SAP97 Polyclonal Antibody | anti-SAP97 antibody

Anti-SAP97 Antibody

Cat. Num. AAA178053

• Gene Names: DLG1; hdlg; DLGH1; SAP97; SAP-97; dJ1061C18.1.1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: SAP97; Polyclonal Antibody; Anti-SAP97 Antibody; Disks large homolog 1; Discs large homolog 1; discs large; Drosophila; homolog of; 1; discs; large homolog 1 (Drosophila); dJ1061C18.1.1; DKFZp761P0818; DKFZp781B0426; DLG 1; DLG1; DLG1_HUMAN; DLGH 1; DLGH1; HDLG; Presynaptic protein SAP97; SAP 97; SAP-97; Synapse associated protein 97; Synapse-associated protein 97; anti-SAP97 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 904

• Applicable Applications for anti-SAP97 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot; Concentration: 0.1-0.5ug/ml; Tested Species: Hu, MS, Rat; ; Immunohistochemistry (Paraffin-embedded Section); Concentration: 0.5-1ug/ml; Tested Species: Hu; Antigen Retrieval: By Heat; ; Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. ; Other applications have not been tested.; Optimal dilutions should be determined by end users.
• Immunogen: E Coli-derived human SAP97 recombinant protein (Position: M1-A165). Human SAP97 shares 89.7% and 84.2% amino acid (aa) sequence identity with mouse and rat SAP97, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- SAP97 Picoband antibody, MBS178053, Western blottingAll lanes: Anti SAP97 (MBS178053) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Mouse Lung Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: MM231 Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: NIH3T3 Whole Cell Lysate at 40ugPredicted bind size: 130KDObserved bind size: 130KD )

Immunohistochemistry (IHC)

(Anti- SAP97 Picoband antibody, MBS178053, IHC(P)IHC(P): Human Prostatic Cancer Tissue )

Related Product Information for anti-SAP97 antibody

Description: Rabbit IgG polyclonal antibody for Disks large homolog 1(DLG1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Disks large homolog 1 (DLG1), also known as synapse-associated protein 97 or SAP97, is a protein that in humans is encoded by the SAP97 gene. SAP97 is expressed throughout the body in epithelial cells, including the kidney and brain. There is some evidence that SAP97 regulates cell-to-cell adhesion during cell death, and may interact with HPV. In the brain, SAP97's function is involved in the trafficking of transmembrane receptors from the ER to the plasma membrane. SAP97's function has been investigated by reducing its expression by knockout or increasing its expression heterologously. Mice in which the SAP97 gene has been knocked out die perinatally, have a cleft palate, and deficiencies in renal function. Overexpression of SAP97 in mammalian neurons leads to increased synaptic strength.

References

1. Müller BM; Kistner U; Veh RW et al. (March 1995). "Molecular characterization and spatial distribution of SAP97, a novel presynaptic protein homologous to SAP90 and the Drosophila discs-large tumor suppressor protein". J. Neurosci. 15 (3 Pt 2): 2354-66. 2. umbaugh G, Sia GM, Garner CC, Huganir RL (June 2003). "Synapse-associated protein-97 isoform-specific regulation of surface AMPA receptors and synaptic function in cultured neurons". J. Neurosci. 23 (11): 4567-76. 3. Sans N, Racca C, Petralia RS, Wang YX, McCallum J, Wenthold RJ (October 2001). "Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway". J. Neurosci. 21 (19): 7506-16.

NCBI and Uniprot Product Information

• NCBI GI #: 148539628
• NCBI GeneID: 1739
• NCBI Accession #: NP_001091894.1
• NCBI GenBank Nucleotide #: NM_001098424.1
• UniProt Accession #: Q12959; Q12958; A5YKK7; B4DGU1; B4DGZ8; B7ZMM0; B9EIQ5; D3DXB8; D3DXB9; E7EWL7; E9PG21
• Molecular Weight: 88,485 Da
• NCBI Official Full Name: disks large homolog 1 isoform 1
• NCBI Official Synonym Full Names: discs large homolog 1, scribble cell polarity complex component
• NCBI Official Symbol: DLG1
• NCBI Official Synonym Symbols: hdlg; DLGH1; SAP97; SAP-97; dJ1061C18.1.1
• NCBI Protein Information: disks large homolog 1
• UniProt Protein Name: Disks large homolog 1
• UniProt Gene Name: DLG1
• UniProt Synonym Gene Names: SAP-97; SAP97
• UniProt Entry Name: DLG1_HUMAN

NCBI Description

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known. [provided by RefSeq, Feb 2011]

Uniprot Description

Essential multidomain scaffolding protein required for normal development (). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.