Immunofluorescence (IF)
(MBS9606664 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit PAR4 Polyclonal Antibody | anti-PAR4 antibody
PAR4 Antibody
Gene Names
F2RL3; PAR4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
PAR4; Polyclonal Antibody; PAR4 Antibody; Coagulation factor II (thrombin) receptor like 3; Coagulation factor II receptor like 3; Coagulation factor II receptor-like 3; F2RL3; PAR 4; PAR-4; PAR4_HUMAN; protease activated receptor 4; protease activated receptor4; Proteinase activated receptor 4; Proteinase-activated receptor 4; Thrombin receptor like 3; Thrombin receptor-like 3; anti-PAR4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PAR4 antibody detects endogenous levels of total PAR4
Purity/Purification
Peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
385
Applicable Applications for anti-PAR4 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA: 1:20000-1:40000
The optimal dilutions should be determined by the end user.
Important Note: For western blots , incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night.
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA: 1:20000-1:40000
The optimal dilutions should be determined by the end user.
Important Note: For western blots , incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night.
Immunogen
A synthesized peptide derived from human PAR4, corresponding to a region within the N-teminal amino acids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9606664 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9606664 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells using PAR4 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells using PAR4 antibody.)
Related Product Information for anti-PAR4 antibody
Function: Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41 KD
NCBI Official Full Name
proteinase-activated receptor 4 preproprotein
NCBI Official Synonym Full Names
F2R like thrombin or trypsin receptor 3
NCBI Official Symbol
F2RL3
NCBI Official Synonym Symbols
PAR4
NCBI Protein Information
proteinase-activated receptor 4
UniProt Protein Name
Proteinase-activated receptor 4
UniProt Gene Name
F2RL3
UniProt Synonym Gene Names
PAR4; PAR-4
NCBI Description
This gene encodes a member of the protease-activated receptor subfamily, part of the G-protein coupled receptor 1 family of proteins. The encoded receptor is proteolytically processed to reveal an extracellular N-terminal tethered ligand that binds to and activates the receptor. This receptor plays a role in blood coagulation, inflammation and response to pain. Hypomethylation at this gene may be associated with lung cancer in human patients. [provided by RefSeq, Sep 2016]
Uniprot Description
Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.
Research Articles on PAR4
Similar Products
Product Notes
The PAR4 f2rl3 (Catalog #AAA9606664) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAR4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAR4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF: 1:100-1:500ICC: 1:100-1:500
ELISA: 1:20000-1:40000
The optimal dilutions should be determined by the end user. Important Note: For western blots, incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night. Researchers should empirically determine the suitability of the PAR4 f2rl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAR4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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