anti-Mouse IL7 Polyclonal Antibody | anti-IL7 antibody

Anti-IL7 Antibody

Cat. Num. AAA177787

• Gene Names: Il7; Il-7; hlb368; A630026I06Rik
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: IL7; Polyclonal Antibody; Anti-IL7 Antibody; Interleukin-7; IL 7; IL-7; Il7; Interleukin 7; interleukin 7; anti-IL7 antibody

• Reactivity: Mouse
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 154

• Applicable Applications for anti-IL7 antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: E Coli-derived mouse IL7 recombinant protein (Position: E26-I154). Mouse IL7 shares 56.4% and 87.6% amino acid (aa) sequence identity with human and rat IL7, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of IL7 expression in mouse brain extract (lane 1), mouse thymus extract (lane 2) and NIH3T3 whole cell lysates (lane 3). IL7 at 20KD was detected using rabbit anti- IL7 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Related Product Information for anti-IL7 antibody

Description: Rabbit IgG polyclonal antibody for Interleukin-7(IL7) detection. Tested with WB in Mouse.

Background: Interleukin 7 (IL-7) is a protein that in humans is encoded by the IL7 gene. The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. And it is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. In addition, this cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.

References

1. Goodwin RG, Lupton S, Schmierer A, Hjerrild KJ, Jerzy R, Clevenger W, Gillis S, Cosman D, Namen AE (January 1989). "Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells". Proc. Natl. Acad. Sci. U.S.A. 86 (1): 302-6. 2. Lupton SD, Gimpel S, Jerzy R, et al. (1990). "Characterization of the human and murine IL-7 genes". J. Immunol. 144 (9): 3592-601. 3. Sutherland GR, Baker E, Fernandez KE, Callen DF, Goodwin RG, Lupton S, Namen AE, Shannon MF, Vadas MA (July 1989). "The gene for human interleukin 7 (IL7) is at 8q12-13". Hum. Genet. 82 (4): 371-2.

NCBI and Uniprot Product Information

• NCBI GI #: 6680433
• NCBI GeneID: 16196
• NCBI Accession #: NP_032397.1
• NCBI GenBank Nucleotide #: NM_008371.5
• UniProt Accession #: P10168
• Molecular Weight: 17,727 Da
• NCBI Official Full Name: interleukin-7 isoform a
• NCBI Official Synonym Full Names: interleukin 7
• NCBI Official Symbol: Il7
• NCBI Official Synonym Symbols: Il-7; hlb368; A630026I06Rik
• NCBI Protein Information: interleukin-7
• UniProt Protein Name: Interleukin-7
• Protein Family: Interleukin
• UniProt Gene Name: Il7
• UniProt Synonym Gene Names: Il-7; IL-7
• UniProt Entry Name: IL7_MOUSE

NCBI Description

The protein encoded by this gene is a hematopoietic growth factor important for B and T cell development. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cell cycle regulation; Cytokine; Cell development/differentiation; Apoptosis; Secreted

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity; growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-7 receptor binding

Biological Process: bone resorption; cell-cell signaling; homeostasis of number of cells within a tissue; humoral immune response; immune response; negative regulation of catalytic activity; organ morphogenesis; positive regulation of B cell proliferation; positive regulation of organ growth; positive regulation of T cell differentiation; regulation of gene expression; T cell lineage commitment