Human Granzyme B ELISA Kit | GZMB elisa kit

Human Granzyme B PicoKine ELISA Kit

Cat. Num. AAA177361

• Gene Names: GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Reactivity: Human
• Synonyms: Granzyme B; Human Granzyme B PicoKine ELISA Kit; granzyme B (granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1); C11; Cathepsin G like 1; CTLA 1; CTSGL1; Cytotoxic T lymphocyte proteinase 2; EC 3.4.21.79; Fragmentin 2; Granzyme 2; HLP; Human lymphocyte protein; Lymphocyte protease; SECT; T cell serine protease 1 3E; GZMB elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human GZMB
• Sequence Length: 247

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GZMB elisa kit

Principle of the Assay: The Picokine™ Human GZMB Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human GZMB with a 96-well strip plate that is pre-coated with antibody specific for GZMB. The detection antibody is a iotinylated antibody specific for GZMB. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human GZMB with immunogen: Expression system for standard: NSO; Immunogen sequence: I21-Y247. The kit is analytically validated with ready to use reagents. To measure Human GZMB, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human GZMB in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human GZMB in the sample.

Background/Introduction: Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes(CTL) and natural killer(NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

NCBI and Uniprot Product Information

• NCBI GI #: 317373361
• NCBI GeneID: 3002
• UniProt Accession #: P10144; Q8N1D2; Q9UCC1
• Molecular Weight: 27,716 Da
• NCBI Official Full Name: Granzyme B
• NCBI Official Synonym Full Names: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2
• UniProt Protein Name: Granzyme B
• Protein Family: Granzyme
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
• UniProt Entry Name: GRAB_HUMAN

NCBI Description

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Protease; EC 3.4.21.79; Apoptosis

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; immunological synapse; nucleus; cytosol

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; natural killer cell mediated cytotoxicity; apoptosis; cytolysis; immune response; protein processing; proteolysis