Typical Testing Data/Standard Curve (for reference only)
(Mouse DLL4 PicoKine ELISA Kit standard curve )
Mouse DLL4 ELISA Kit | DLL4 elisa kit
Mouse DLL4 PicoKine ELISA Kit
Gene Names
Dll4; Delta4
Reactivity
Mouse
Synonyms
DLL4; Mouse DLL4 PicoKine ELISA Kit; Delta-like protein 4; delta-like 4 (Drosophila); Delta 4; delta 4 precursor; Delta ligand 4; delta ligand 4 precursor; Delta like 4; Delta like 4 homolog; Delta like 4 protein; Delta like protein 4; Delta-like 4 (Drosophila); Delta4; DLL 4; DLL4_HUMAN; Drosophila Delta homolog 4; hdelta2; Homeobox protein DLL-4; MGC126344; Notch ligand delta 2; Notch ligand DLL4; notch ligand DLL4 precursor; XDLL-4; DLL4 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse DLL4
Sequence Length
686
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse DLL4 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse DLL4 PicoKine ELISA Kit standard curve )
Related Product Information for DLL4 elisa kit
Principle of the assay: mouse DLL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DLL4 has been precoated onto 96-well plates. Standards(NSO, S28-P525) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DLL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DLL4 amount of sample captured in plate.
Background: Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. Mouse DLL4 could activate mouse Notch1 and mouse Notch4. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.
Background: Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. Mouse DLL4 could activate mouse Notch1 and mouse Notch4. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
74,991 Da
NCBI Official Full Name
Delta-like protein 4
NCBI Official Synonym Full Names
delta-like 4 (Drosophila)
NCBI Official Symbol
Dll4
NCBI Official Synonym Symbols
Delta4
NCBI Protein Information
delta-like protein 4; drosophila Delta homolog 4
UniProt Protein Name
Delta-like protein 4
Protein Family
UniProt Gene Name
Dll4
UniProt Synonym Gene Names
Delta4
UniProt Entry Name
DLL4_MOUSE
Uniprot Description
DLL4: Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4. Binds to Notch-1 and Notch-4. Expressed in vascular endothelium.
Protein type: Cell development/differentiation; Membrane protein, integral
Cellular Component: membrane; integral to plasma membrane; integral to membrane
Molecular Function: protein binding; calcium ion binding; Notch binding
Biological Process: nervous system development; Notch signaling pathway; multicellular organismal development; response to stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of blood vessel endothelial cell migration; patterning of blood vessels; negative regulation of cell proliferation; regulation of neurogenesis; visual perception; blood vessel remodeling; cell communication; angiogenesis; cell differentiation
Research Articles on DLL4
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Product Notes
The Mouse DLL4 dll4 (Catalog #AAA177248) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177248 ELISA Kit recognizes Mouse DLL4. It is sometimes possible for the material contained within the vial of "DLL4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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