Western Blot (WB)
(Figure 1: Western blot analysis using DLL4 mAb against human DLL4 (AA: 313-439) recombinant protein. (Expected MW is 39.2 kDa))
Mouse anti-Human DLL4 Monoclonal Antibody | anti-DLL4 antibody
DLL4 Antibody
Gene Names
DLL4; AOS6; delta4; hdelta2
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Affinity-Chromatography
Synonyms
DLL4; Monoclonal Antibody; DLL4 Antibody; AOS6; Delta 4; delta 4 precursor; Delta ligand 4; delta ligand 4 precursor; Delta like 4; Delta like 4 homolog; Delta like 4 protein; Delta like canonical Notch ligand 4; Delta like protein 4; Delta-like 4 (Drosophila); Delta-like protein 4; Delta4; DLL 4; Dll4; DLL4_HUMAN; Drosophila Delta homolog 4; hdelta2; Homeobox protein DLL-4; MGC126344; Notch ligand delta 2; Notch ligand DLL4; Notch ligand DLL4 precursor; XDLL-4; anti-DLL4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
DLL4 antibody detects endogenous levels of total DLL4
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
685
Applicable Applications for anti-DLL4 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
FC/FACS: 1:200-1:400
WB: 1:500-1:2000
FC/FACS: 1:200-1:400
Immunogen
Purified recombinant fragment of human DLL4 expressed in E. Coli
Subcellular Location
Membrane.
Tissue Specificity
Expressed in vascular endothelium.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using DLL4 mAb against human DLL4 (AA: 313-439) recombinant protein. (Expected MW is 39.2 kDa))
Western Blot (WB)
(Figure 1: Western blot analysis using DLL4 mAb against human DLL4 (AA: 313-439) recombinant protein. (Expected MW is 39.2 kDa))
Related Product Information for anti-DLL4 antibody
Description: This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.
Function: Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed:20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed:17728344).
Subunit Structure: Interacts with NOTCH4. Interacts (via N-terminal DSL and MNNL domains) with NOTCH1 (via EGF-like domains).
Similarity: The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
Function: Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed:20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed:17728344).
Subunit Structure: Interacts with NOTCH4. Interacts (via N-terminal DSL and MNNL domains) with NOTCH1 (via EGF-like domains).
Similarity: The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 74.6 kDa
Predicted: 75 kDa
Predicted: 75 kDa
NCBI Official Full Name
delta-like protein 4
NCBI Official Synonym Full Names
delta like canonical Notch ligand 4
NCBI Official Symbol
DLL4
NCBI Official Synonym Symbols
AOS6; delta4; hdelta2
NCBI Protein Information
delta-like protein 4
UniProt Protein Name
Delta-like protein 4
Protein Family
UniProt Gene Name
DLL4
UniProt Synonym Gene Names
Delta4
NCBI Description
This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. [provided by RefSeq, Jul 2008]
Uniprot Description
Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed:20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed:17728344).
Research Articles on DLL4
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Product Notes
The DLL4 dll4 (Catalog #AAA9602587) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DLL4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DLL4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:10000 WB: 1:500-1:2000 FC/FACS: 1:200-1:400. Researchers should empirically determine the suitability of the DLL4 dll4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLL4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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