Immunohistochemistry (IHC)
(Anti- CXCR6 antibody, MBS176980, IHC(P)IHC(P): Rat Spleen Tissue )
Rabbit anti-Mouse, Rat Bonzo Polyclonal Antibody | anti-CXCR6 antibody
Anti-Bonzo antibody
Gene Names
Cxcr6; BONZO; STRL33; BB217514
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
Bonzo; Polyclonal Antibody; Anti-Bonzo antibody; C-X-C chemokine receptor type 6; chemokine(C-X-C motif) receptor 6; C X C chemokine receptor type 6 antibody; C-X-C chemokine receptor type 6 antibody; CD 186 antibody; CD186 antibody; CD186 antigen antibody; CDw186 antibody; Chemokine(C X C motif) receptor 6 antibody; Chemokine C X C motif receptor 6 antibody; Chemokine C-X-C Motif Receptor 6 antibody; Chemokine CXC motif receptor 6 antibody; Chemokine receptor 6 antibody; CXC chemokine receptor type 6 antibody; CXC R6 antibody; CXC-R6 antibody; CXCR 6 antibody; CXCR-6 antibody; CXCR6 antibody; CXCR6_HUMAN antibody; G protein coupled receptor antibody; G protein coupled receptor bonzo antibody; G protein coupled receptor STRL 33 antibody; G protein coupled receptor STRL33 antibody; G protein coupled receptor TYMSTR antibody; G-protein coupled receptor bonzo antibody; G-protein coupled receptor STRL33 antibody; STRL 33 antibody; STRL33 antibody; TYMSTR antibody; anti-CXCR6 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
351
Applicable Applications for anti-CXCR6 antibody
Western Blot (WB), Immunohistochemistry (IHC-P) Paraffin
Application Notes
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse, Rat
Immunohistochemistry (Paraffin):
Concentration - 0.5-1ug/ml
Tested Species: Rat
Predicted Species: Mouse
Antigen Revival: By Heat
Tested Species: In-house tested species with positive results
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling paraffin sections in 10 mM cirate buffer, pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Other dilutions should be determined by end users.
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse, Rat
Immunohistochemistry (Paraffin):
Concentration - 0.5-1ug/ml
Tested Species: Rat
Predicted Species: Mouse
Antigen Revival: By Heat
Tested Species: In-house tested species with positive results
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling paraffin sections in 10 mM cirate buffer, pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Other dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bonzo (331-351aa EDSSKTCSASHNVETTSMFQL), different from the related rat sequence by two amino acids, and from the related human sequence by three amino acids
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cross-Reactivity
No cross reactivity with other proteins.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Anti- CXCR6 antibody, MBS176980, IHC(P)IHC(P): Rat Spleen Tissue )
Immunohistochemistry (IHC)
(Anti- CXCR6 antibody, MBS176980, IHC(P)IHC(P): Rat Spleen Tissue )
Western Blot (WB)
(Anti- CXCR6 antibody, MBS176980, Western blottingAll lanes: Anti CXCR6 (MBS176980) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Testis Tissue Lysate at 50ugLane 3: Rat Spleen Tissue Lysate at 50ugLane 4: Mouse Brain Tissue Lysate at 50ugLane 5: Mouse Testis Tissue Lysate at 50ugPredicted bind size: 39KDObserved bind size: 39KD )
Western Blot (WB)
(Anti- CXCR6 antibody, MBS176980, Western blottingAll lanes: Anti CXCR6 (MBS176980) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Testis Tissue Lysate at 50ugLane 3: Rat Spleen Tissue Lysate at 50ugLane 4: Mouse Brain Tissue Lysate at 50ugLane 5: Mouse Testis Tissue Lysate at 50ugPredicted bind size: 39KDObserved bind size: 39KD )
Related Product Information for anti-CXCR6 antibody
Description: Rabbit IgG polyclonal antibody for C-X-C chemokine receptor type 6(CXCR6) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: CXCR6(Chemokine,CXC Motif, Receptor 6), also known as STRL33, is a protein that in humans is encoded by the CXCR6 gene. By Southern blot analysis of genomic DNA and somatic cell hybrid analysis, Liao et al.(1997) mapped the single-copy STRL33 gene to chromosome 3. Matloubian et al.(2000) found that human and mouse cells expressing CXCR6 showed a strong chemotactic response to CXCL16 but not to other chemokines. The authors concluded that CXCL16 and CXCR6 probably function in interactions between dendritic cells and T cells and in regulating T-cell migration in the splenic red pulp. Kim et al.(2001) concluded that CXCR6 may be important in the trafficking of effector T cells mediating type-1 inflammation.
Background: CXCR6(Chemokine,CXC Motif, Receptor 6), also known as STRL33, is a protein that in humans is encoded by the CXCR6 gene. By Southern blot analysis of genomic DNA and somatic cell hybrid analysis, Liao et al.(1997) mapped the single-copy STRL33 gene to chromosome 3. Matloubian et al.(2000) found that human and mouse cells expressing CXCR6 showed a strong chemotactic response to CXCL16 but not to other chemokines. The authors concluded that CXCL16 and CXCR6 probably function in interactions between dendritic cells and T cells and in regulating T-cell migration in the splenic red pulp. Kim et al.(2001) concluded that CXCR6 may be important in the trafficking of effector T cells mediating type-1 inflammation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
C-X-C chemokine receptor type 6
NCBI Official Synonym Full Names
chemokine (C-X-C motif) receptor 6
NCBI Official Symbol
Cxcr6
NCBI Official Synonym Symbols
BONZO; STRL33; BB217514
NCBI Protein Information
C-X-C chemokine receptor type 6; CXC-R6; CXCR-6
UniProt Protein Name
C-X-C chemokine receptor type 6
Protein Family
UniProt Gene Name
Cxcr6
UniProt Synonym Gene Names
CXC-R6; CXCR-6
UniProt Entry Name
CXCR6_MOUSE
Uniprot Description
CXCR6: Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass
Cellular Component: membrane; integral to membrane; plasma membrane
Molecular Function: C-X-C chemokine binding; G-protein coupled receptor activity; signal transducer activity; chemokine receptor activity; coreceptor activity; C-X-C chemokine receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; chemotaxis; signal transduction; inflammatory response
Research Articles on CXCR6
Similar Products
Product Notes
The CXCR6 cxcr6 (Catalog #AAA176980) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Bonzo antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bonzo can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC-P) Paraffin. Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Mouse, Rat Immunohistochemistry (Paraffin): Concentration - 0.5-1ug/ml Tested Species: Rat Predicted Species: Mouse Antigen Revival: By Heat Tested Species: In-house tested species with positive results Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling paraffin sections in 10 mM cirate buffer, pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections. Other applications have not been tested. Other dilutions should be determined by end users. Researchers should empirically determine the suitability of the CXCR6 cxcr6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bonzo, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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