Mouse CXCL13/BLC ELISA Kit | CXCL13 elisa kit

Mouse CXCL13/BLC PicoKine ELISA Kit

Cat. Num. AAA176540

• Gene Names: Cxcl13; BLC; Angie; BCA-1; BLR1L; ANGIE2; Scyb13; 4631412M08Rik
• Reactivity: Mouse
• Synonyms: CXCL13/BLC; Mouse CXCL13/BLC PicoKine ELISA Kit; C-X-C motif chemokine 13; chemokine (C-X-C motif) ligand 13; ANGIE; ANGIE2; B cell attracting chemokine 1; B cell chemoattractant; B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1); B lymphocyte chemoattractant; BCA 1; BLC; BLR1L; C X C motif chemokine 13; Chemokine (C X C motif) ligand 13; Chemokine (C X C motif) ligand 13 (B cell chemoattractant); Chemokine CXC motif ligand 13; CXC chemokine BLC; CXCL13; SCYB13; Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant); Small inducible cytokine B13; Small inducible cytokine subfamily B; member 13; CXCL13 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CXCL13
• Sequence Length: 109

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse CXCL13/BLC PicoKine ELISA Kit standard curve )

Related Product Information for CXCL13 elisa kit

Principle of the assay: mouse CXCL13 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL13 has been precoated onto 96-well plates. Standards (E.coli,I22-A109) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL13 amount of sample captured in plate.
Background: C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.

NCBI and Uniprot Product Information

• NCBI GI #: 7673946
• NCBI GeneID: 55985
• UniProt Accession #: O55038
• Molecular Weight: 11,927 Da
• NCBI Official Full Name: C-X-C motif chemokine 13
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 13
• NCBI Official Symbol: Cxcl13
• NCBI Official Synonym Symbols: BLC; Angie; BCA-1; BLR1L; ANGIE2; Scyb13; 4631412M08Rik
• NCBI Protein Information: C-X-C motif chemokine 13; CXC chemokine BLC; B lymphocyte chemoattractant; small-inducible cytokine B13; small inducible cytokine subfamily B (Cys-X-Cys), member 13
• UniProt Protein Name: C-X-C motif chemokine 13
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: Cxcl13
• UniProt Synonym Gene Names: Blc; Scyb13
• UniProt Entry Name: CXL13_MOUSE

Uniprot Description

CXCL13: Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: heparin binding; fibroblast growth factor binding; CXCR3 chemokine receptor binding; chemokine activity; protein heterodimerization activity; CCR10 chemokine receptor binding; CXCR5 chemokine receptor binding; cytokine activity; receptor agonist activity

Biological Process: lymphocyte chemotaxis across high endothelial venule; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; chemotaxis; positive regulation of integrin activation; positive regulation of cell-cell adhesion mediated by integrin; lymph node development; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; defense response to bacterium; immune response; inflammatory response