Human CXCL13 ELISA Kit | CXCL13 elisa kit

Human CXCL13 ELISA Kit

Cat. Num. AAA824727

• Gene Names: CXCL13; BLC; BCA1; ANGIE; BCA-1; BLR1L; ANGIE2; SCYB13
• Reactivity: Human
• Applications: ELISA
• Synonyms: CXCL13; Human CXCL13 ELISA Kit; BCA1; BLC; SCYB13; C-X-C motif chemokine 13; Angie; B cell-attracting chemokine 1; BCA-1; B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13; CXCL13 elisa kit

• Reactivity: Human
• Specificity: The Human CXCL13 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CXCL13 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 109

• Applicable Applications for CXCL13 elisa kit: Sandwich ELISA (SE)
• Samples: Cell culture supernatants, serum, plasma
• Sensitivity: < 8 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CXCL13 elisa kit

Principle of the Assay: The MyBioSource' Human CXCL13 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL13 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CXCL13 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the Tcell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.

NCBI and Uniprot Product Information

• NCBI GI #: 5453577
• NCBI GeneID: 10563
• NCBI Accession #: NP_006410.1
• NCBI GenBank Nucleotide #: NM_006419.2
• UniProt Accession #: O43927
• Molecular Weight: 12,664 Da
• NCBI Official Full Name: C-X-C motif chemokine 13
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 13
• NCBI Official Symbol: CXCL13
• NCBI Official Synonym Symbols: BLC; BCA1; ANGIE; BCA-1; BLR1L; ANGIE2; SCYB13
• NCBI Protein Information: C-X-C motif chemokine 13; CXC chemokine BLC; B-cell chemoattractant; B-lymphocyte chemoattractant; b lymphocyte chemoattractant; small-inducible cytokine B13; B-cell-attracting chemokine 1; b cell-attracting chemokine 1; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)
• UniProt Protein Name: C-X-C motif chemokine 13
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL13
• UniProt Synonym Gene Names: BCA1; BLC; SCYB13; BCA-1
• UniProt Entry Name: CXL13_HUMAN

NCBI Description

B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq, Oct 2014]

Uniprot Description

CXCL13: Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; fibroblast growth factor binding; CXCR3 chemokine receptor binding; chemokine activity; protein heterodimerization activity; CCR10 chemokine receptor binding; CXCR5 chemokine receptor binding; receptor agonist activity

Biological Process: chronic inflammatory response; lymphocyte chemotaxis across high endothelial venule; response to lipopolysaccharide; lymph node development; positive regulation of integrin activation; positive regulation of cell-cell adhesion mediated by integrin; regulation of angiogenesis; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; regulation of humoral immune response; defense response to bacterium; germinal center formation; immune response; inflammatory response