Human CXCL13/BLC ELISA Kit | CXCL13 elisa kit

Human CXCL13/BLC PicoKine ELISA Kit

Cat. Num. AAA176488

• Gene Names: CXCL13; BLC; BCA1; ANGIE; BCA-1; BLR1L; ANGIE2; SCYB13
• Reactivity: Human
• Synonyms: CXCL13/BLC; Human CXCL13/BLC PicoKine ELISA Kit; C-X-C motif chemokine 13; chemokine (C-X-C motif) ligand 13; ANGIE; ANGIE2; B cell attracting chemokine 1; B cell chemoattractant; B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1); B lymphocyte chemoattractant; BCA 1; BLC; BLR1L; C X C motif chemokine 13; Chemokine (C X C motif) ligand 13; Chemokine (C X C motif) ligand 13 (B cell chemoattractant); Chemokine CXC motif ligand 13; CXC chemokine BLC; CXCL13; SCYB13; Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant); Small inducible cytokine B13; Small inducible cytokine subfamily B; member 13; CXCL13 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CXCL13
• Sequence Length: 109

• Samples: Cell culture supernates, serum, plasma(heparin, EDTA) and saliva
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CXCL13 elisa kit

Principle of the Assay: The Picokine™ Human CXCL13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CXCL13 with a 96-well strip plate that is pre-coated with antibody specific for CXCL13. The detection antibody is a biotinylated antibody specific for CXCL13. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CXCL13 with immunogen: Expression system for standard: E.coli; Immunogen sequence: V23-R94. The kit is analytically validated with ready to use reagents. To measure Human CXCL13, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CXCL13 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CXCL13 in the sample.!!Background/Introduction: C-X-C motif chemokine 13(CXCL13), also known as B lymphocyte chemoattractant(BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells

NCBI and Uniprot Product Information

• NCBI GI #: 7673945
• NCBI GeneID: 10563
• UniProt Accession #: O43927
• Molecular Weight: 12,664 Da
• NCBI Official Full Name: C-X-C motif chemokine 13
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 13
• NCBI Official Symbol: CXCL13
• NCBI Official Synonym Symbols: BLC; BCA1; ANGIE; BCA-1; BLR1L; ANGIE2; SCYB13
• NCBI Protein Information: C-X-C motif chemokine 13; CXC chemokine BLC; B-cell chemoattractant; B-lymphocyte chemoattractant; b lymphocyte chemoattractant; small-inducible cytokine B13; B-cell-attracting chemokine 1; b cell-attracting chemokine 1; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)
• UniProt Protein Name: C-X-C motif chemokine 13
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL13
• UniProt Synonym Gene Names: BCA1; BLC; SCYB13; BCA-1
• UniProt Entry Name: CXL13_HUMAN

NCBI Description

B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq, Oct 2014]

Uniprot Description

CXCL13: Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; fibroblast growth factor binding; chemokine activity; protein heterodimerization activity; CXCR5 chemokine receptor binding; CCR10 chemokine receptor binding; receptor agonist activity

Biological Process: chronic inflammatory response; lymphocyte chemotaxis across high endothelial venule; response to lipopolysaccharide; lymph node development; positive regulation of integrin activation; regulation of angiogenesis; positive regulation of cell-cell adhesion mediated by integrin; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; regulation of humoral immune response; defense response to bacterium; germinal center formation; immune response; inflammatory response