Rabbit MBD4 Polyclonal Antibody | anti-MBD4 antibody
Anti-MBD4 antibody
Western Blot (WB)
(Anti-MBD4 antibody, MBS176323, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human MBD4, 39.7KD(162aa tag+ Q400-S580)Lane 1: Recombinant Human MBD4 Protein 10ngLane 2: Recombinant Human MBD4 Protein 5ngLane 3: Recombinant Human MBD4 Protein 2.5ng)
Background: MBD4(Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells(Hendrich and Bird, 1998). Riccio et al.(1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird(1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al.(1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
Uniprot Description
MBD4: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; Deoxyribonuclease; EC 3.2.2.-; Apoptosis
Chromosomal Location of Human Ortholog: 3q21.3
Cellular Component: nucleoplasm; cytoplasm; nucleus; chromatin
Molecular Function: protein binding; satellite DNA binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; endodeoxyribonuclease activity
Biological Process: response to radiation; DNA damage response, signal transduction resulting in induction of apoptosis; base-excision repair, AP site formation; depyrimidination; base-excision repair; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA repair; DNA catabolic process, endonucleolytic