Rabbit anti-Human SUCLG2 Polyclonal Antibody | anti-SUCLG2 antibody

SUCLG2 Antibody

Cat. Num. AAA7108475

• Gene Names: SUCLG2; G-SCS; GBETA; GTPSCS
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: SUCLG2; Polyclonal Antibody; SUCLG2 Antibody; Succinate--CoA ligase [GDP-forming] subunit beta; mitochondrial; GTP-specific succinyl-CoA synthetase subunit beta; anti-SUCLG2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 440

• Applicable Applications for anti-SUCLG2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:5000; IHC: 1:200-1:500
• Immunogen: Recombinant Human Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial protein (161-292AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, Raji whole cell lysate, LO2 whole cell lysate, U87 whole cell lysate, All lanes: SUCLG2 antibody at 6.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47, 48 KDaObserved band size: 47 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7108475 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of MBS7108475 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Related Product Information for anti-SUCLG2 antibody

GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.

Product Categories/Family for anti-SUCLG2 antibody

Metabolism; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 294862256
• NCBI GeneID: 8801
• NCBI Accession #: NP_001171070.1
• NCBI GenBank Nucleotide #: NM_001177599.1
• UniProt Accession #: Q96I99; O95195; Q6NUH7; Q86VX8; Q8WUQ1; C9JVT2
• Molecular Weight: 47,732 Da
• NCBI Official Full Name: succinate--CoA ligase
• NCBI Official Synonym Full Names: succinate-CoA ligase GDP-forming beta subunit
• NCBI Official Symbol: SUCLG2
• NCBI Official Synonym Symbols: G-SCS; GBETA; GTPSCS
• NCBI Protein Information: succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial; succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial
• UniProt Protein Name: Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
• Protein Family: Succinate--CoA ligase
• UniProt Gene Name: SUCLG2
• UniProt Synonym Gene Names: G-SCS; GTPSCS; SCS-betaG

NCBI Description

This gene encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 5 and 12. [provided by RefSeq, Apr 2010]

Uniprot Description

GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.