Rabbit Hydroxysteroid(17-beta) Dehydrogenase 4 Polyclonal Antibody | anti-HSD17B4 antibody

Anti-Hydroxysteroid(17-beta) Dehydrogenase 4 antibody

Cat. Num. AAA175708

• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Hydroxysteroid(17-beta) Dehydrogenase 4; Polyclonal Antibody; Anti-Hydroxysteroid(17-beta) Dehydrogenase 4 antibody; Enoyl-CoA hydratase 2; hydroxysteroid(17-beta) dehydrogenase 4; 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody; 17 beta HSD 4 antibody; 17 beta HSD IV antibody; 17 beta hydroxysteroid dehydrogenase 4 antibody; 17-beta-HSD 4 antibody; 17-beta-hydroxysteroid dehydrogenase 4 antibody; 17beta estradiol dehydrogenase type IV antibody; 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase antibody; 3-alpha antibody; 7-alpha antibody; Beta hydroxyacyl dehydrogenase antibody; Beta keto reductase antibody; D 3 hydroxyacyl CoA dehydratase antibody; D bifunctional protein antibody; D bifunctional protein peroxisomal antibody; D-bifunctional protein antibody; DBP antibody; DHB4_HUMAN antibody; EDH17B4 antibody; Enoyl-CoA hydratase 2 antibody; Hsd17b4 antibody; MFE 2 antibody; MFE-2 antibody; MPF-2 antibody; Multifunctional protein 2 antibody; Peroxisomal multifunctional enzyme type 2 antibody; Peroxisomal multifunctional protein 2 antibody; SDR8C1 antibody; Short chain dehydrogenase/reductase family 8C member 1 antibody; anti-HSD17B4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA,0.9mg NaCI, 0.2 mg Na₂HPO₄, 0.05 mg Thimerosal, 0.05 mg NaN₃
• Sequence Length: 718

• Applicable Applications for anti-HSD17B4 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot (WB); ; Concentration: 0.1-0.5 ug/ml; Tested Species: Hu,Rat; Concluded Species : Ms; ; ; Immunohistochemistry-Paraffin (IHC-P); ; Cocentration: 0.5-1 ug/ml; Tested Speices: Hu; Concluded Species: Rat, Ms; Antigen Retrieval: By Heat; ; ; Other applications have not been tested.; ; Optimal dilutions should be determined by end user.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human HSD17B4(744-761aa NIMLSQKLQMILKDYAKL), identical to the related rat and mouse sequences.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4° C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Hydroxysteroid(17-beta) Dehydrogenase 4 antibody, MBS175708, IHC(P)IHC(P): Human Lung Cancer Tissue )

Western Blot (WB)

(Figure 1. Western blot analysis of HSD17B4 using anti- HSD17B4 antibody (MBS175708).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse heart tissue lysates,Lane 2: rat heart tissue lysates,Lane 3: human placenta tissue lysates,Lane 4: MCF-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- HSD17B4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSD17B4 at approximately 80KD. The expected band size for HSD17B4 is at 80KD.)

Immunohistochemistry (IHC)

(Anti-Hydroxysteroid(17-beta) Dehydrogenase 4 antibody, MBS175708, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Related Product Information for anti-HSD17B4 antibody

Description: Rabbit IgG polyclonal antibody for(3R)-hydroxyacyl-CoA dehydrogenase(HSD17B4) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Background: The HSD17B4 gene encodes an enzyme involved in peroxisomal fatty acid beta-oxidation. It was first identified as a 17-beta-estradiol dehydrogenase. Peroxisomal beta-oxidation of fatty acids is catalyzed by 3 enzymes: acyl-CoA oxidase; the D-bifunctional enzyme with enoyl-CoA-hydratase and D-3-hydroxyacyl-CoA dehydrogenase activity, and 3-ketoacyl-CoA thiolase. The D- and L-bifunctional proteins have different substrate specificities. The D-bifunctional protein catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids and also acts in shortening cholesterol for bile acid formation. In contrast, the L-specific bifunctional protein does not have the latter 2 activities.

NCBI and Uniprot Product Information

• UniProt Accession #: F5HE57; B4DNV1; B4DVS5; E9PB82
• UniProt Protein Name: Peroxisomal multifunctional enzyme type 2
• UniProt Gene Name: HSD17B4
• UniProt Synonym Gene Names: EDH17B4; MFE-2; 17-beta-HSD 4; DBP; MPF-2
• UniProt Entry Name: DHB4_HUMAN

Uniprot Description

HSD17B4: Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. Catalyzes the formation of 3- ketoacyl-CoA intermediates from both straight-chain and 2-methyl- branched-chain fatty acids. Defects in HSD17B4 are a cause of D-bifunctional protein deficiency (DBPD). DBPD is a disorder of peroxisomal fatty acid beta-oxidation. Defects in HSD17B4 are the cause of Perrault syndrome (PRS). PRS is a sex-influenced disorder characterized by sensorineural deafness in both males and females and ovarian dysgenesis in females. Some patients also have neurologic manifestations, including mild mental retardation and cerebellar and peripheral nervous system involvement. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: EC 1.1.1.n12; Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; EC 4.2.1.119; Mitochondrial; EC 4.2.1.107; Lyase; Cell development/differentiation

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: peroxisomal membrane; peroxisomal matrix; intracellular membrane-bound organelle; membrane; mitochondrion; peroxisome

Molecular Function: protein homodimerization activity; 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity; long-chain-enoyl-CoA hydratase activity; isomerase activity; 3-hydroxyacyl-CoA dehydrogenase activity; receptor binding

Biological Process: osteoblast differentiation; bile acid biosynthetic process; estrogen metabolic process; fatty acid beta-oxidation using acyl-CoA oxidase; fatty acid beta-oxidation; bile acid metabolic process; very-long-chain fatty acid metabolic process; metabolic process; unsaturated fatty acid metabolic process; androgen metabolic process; cellular lipid metabolic process; Sertoli cell development

Disease: Perrault Syndrome 1; D-bifunctional Protein Deficiency