Mouse coagulation factor VII (serum prothrombin conversion accelerator) ELISA Kit | F7 elisa kit

Mouse coagulation factor VII, FVII ELISA Kit

Cat. Num. AAA944525

• Gene Names: F7; Cf7; FVII; mfVII; AI132620
• Reactivity: Mouse
• Synonyms: coagulation factor VII (serum prothrombin conversion accelerator); Mouse coagulation factor VII; FVII ELISA Kit; FVII coagulation protein; coagulation factor VII; eptacog alfa; F7 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse FVII. No significant cross-reactivity or interference between mouse FVII and analogues was observed.
• Sequence Length: 446

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.45 ng/ml-30 ng/ml
• Sensitivity: <0.11 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F7 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FVII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FVII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FVII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FVII bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 225543550
• NCBI GeneID: 14068
• NCBI Accession #: NP_034302.2
• NCBI GenBank Nucleotide #: NM_010172.3
• UniProt Accession #: P70375; Q61109
• Molecular Weight: 50,276 Da
• NCBI Official Full Name: coagulation factor VII
• NCBI Official Synonym Full Names: coagulation factor VII
• NCBI Official Symbol: F7
• NCBI Official Synonym Symbols: Cf7; FVII; mfVII; AI132620
• NCBI Protein Information: coagulation factor VII; serum prothrombin conversion accelerator
• UniProt Protein Name: Coagulation factor VII
• Protein Family: Coagulation factor
• UniProt Gene Name: F7
• UniProt Synonym Gene Names: Cf7
• UniProt Entry Name: FA7_MOUSE

NCBI Description

This gene encodes a vitamin K-dependent serine protease that plays a critical role in the extrinsic pathway of blood coagulation. Upon contact with tissue factor III (TF III), the encoded protein forms an activated complex termed TF-FVIIa that initiates the coagulation cascade involving other coagulation factors, ultimately resulting in a fibrin clot. Complete lack of the encoded protein in mice results in in perinatal lethality due to bleeding from normal blood vessels. [provided by RefSeq, Apr 2015]

Uniprot Description

F7: Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. Defects in F7 are the cause of factor VII deficiency (FA7D). A hemorrhagic disease with variable presentation. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or repeated hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Finally, numerous subjects are completely asymptomatic despite very low factor VII levels. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Apoptosis; EC 3.4.21.21; Protease

Cellular Component: extracellular space; extracellular region; vesicle

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; endopeptidase activity; calcium ion binding; glycoprotein binding; catalytic activity; receptor binding

Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of blood coagulation; positive regulation of positive chemotaxis; hemostasis; positive regulation of leukocyte chemotaxis; blood coagulation; proteolysis