Testing Data
Human MBL2/MPB-C ELISA Kit | MBL2 elisa kit
Human MBL2/MPB-C Quick ELISA Kit
O.D.|0.027|0.388|0.693|1.231|2.064|2.644|2.417|2.577
n|16|16|16|
Mean(pg/ml)|482|2754|8841|br>Standard deviation|26.99|126.68|680.76|
CV(%)|5.6%|4.6%|7.7%|
n|24|24|24
Mean(pg/ml)|446|2879|8912
Standard deviation|33|149.71|730.78
CV(%)|7.4%|5.2%|8.2%
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 482|544|480|477|496|31.23|6.3%
Sample 2: 2754|2813|2929|2845|2835|59.54|2.1%
Sample 3: 8841|9907|8436|9421|9151|503.32|5.5%
*number of samples for each test n=16.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
To measure Human MBL2, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MBL2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MBL2 in the sample.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]
Uniprot Description
MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10q11.2
Cellular Component: extracellular space; cell surface; collagen; extracellular region
Molecular Function: mannose binding; protein binding; calcium ion binding; calcium-dependent protein binding; receptor binding
Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; positive regulation of phagocytosis; defense response to bacterium; acute-phase response; innate immune response; response to oxidative stress; opsonization; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction; complement activation
Disease: Mannose-binding Protein Deficiency
Research Articles on MBL2
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Product Notes
The Human MBL2 mbl2 (Catalog #AAA1753932) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1753932 ELISA Kit recognizes Human MBL2. It is sometimes possible for the material contained within the vial of "MBL2/MPB-C, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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