Human MBL2 ELISA Kit | MBL2 elisa kit

Human MBL2 Binding Protein ELISA Kit

Cat. Num. AAA8244576

• Gene Names: MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• Reactivity: Human
• Applications: ELISA
• Synonyms: MBL2; Human MBL2 Binding Protein ELISA Kit; COLEC1; MBL; Mannose-binding protein C; MBP-C; Collectin-1; MBP1; Mannan-binding protein; Mannose-binding lectin; MBL2 elisa kit

• Reactivity: Human
• Specificity: The Human MBL2 Binding Protein ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MBL2 Binding Protein proteins within the range of 312 pg/ml - 20000 pg/ml.
• Sequence Length: 248

• Applicable Applications for MBL2 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for MBL2 elisa kit

Principle of the Assay||The Human MBL2 Binding Protein ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MBL2 Binding Protein in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human MBL2 Binding Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and MBL2 Binding Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MBL2 Binding Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MBL2 Binding Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 126676
• NCBI GeneID: 4153
• UniProt Accession #: P11226; Q4VB12; Q4VB13; Q4VB14; Q5SQS3; Q86SI4; Q96KE4; Q96TF7; Q96TF8; Q96TF9
• Molecular Weight: 26,144 Da
• NCBI Official Full Name: Mannose-binding protein C
• NCBI Official Synonym Full Names: mannose binding lectin 2
• NCBI Official Symbol: MBL2
• NCBI Official Synonym Symbols: MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• NCBI Protein Information: mannose-binding protein C
• UniProt Protein Name: Mannose-binding protein C
• Protein Family: Mannose-binding protein
• UniProt Gene Name: MBL2
• UniProt Synonym Gene Names: COLEC1; MBL; MBP-C
• UniProt Entry Name: MBL2_HUMAN

NCBI Description

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cell surface; collagen; extracellular region; extracellular space

Molecular Function: calcium ion binding; calcium-dependent protein binding; mannose binding; protein binding; receptor binding

Biological Process: acute-phase response; complement activation; complement activation, classical pathway; complement activation, lectin pathway; defense response to bacterium; defense response to Gram-positive bacterium; innate immune response; killing by host of symbiont cells; negative regulation of viral reproduction; opsonization; positive regulation of phagocytosis; response to oxidative stress

Disease: Mannose-binding Protein Deficiency