Human SPINT1/HAI-1 ELISA Kit | SPINT1 elisa kit

Human SPINT1/HAI-1 Quick ELISA Kit

Cat. Num. AAA1753929

• Gene Names: SPINT1; HAI; HAI1; MANSC2
• Reactivity: Human
• Synonyms: SPINT1/HAI-1; Human SPINT1/HAI-1 Quick ELISA Kit; HAI-1/HGFA Inhibitor 1|HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor; Kunitz type 1; serine protease inhibitor; SPINT1; SPINT1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human SPINT1

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, serum and plasma (heparin)
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <15pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.036|0.102|0.150|0.243|0.421|0.765|1.383|2.228
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|211|1855|3887|; Standard deviation|13.5|116.87|264.32|; CV(%)|6.4%|6.3%|6.8%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|234|1852|3784; Standard deviation|17.55|120.38|298.94; CV(%)|7.5%|6.5%|7.9%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 211|244|208|233|224|12.32|5.5%; Sample 2: 1855|1675|1694|1706|1733|39.85|2.3%; Sample 3: 3887|4423|4263|4699|4318|250.44|5.8%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: G36-L529
• Anticoagulant: heparin
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human SPINT1/HAI-1 with <15pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human SPINT1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human SPINT1 in cell culture supernatants, serum and plasma (heparin). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human SPINT1 with immunogen: Expression system for standard: NS0; Immunogen sequence: G36-L529.; ; To measure Human SPINT1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human SPINT1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human SPINT1 in the sample.
• Assay Procedure Step 1: 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312.5pg/ml, 156.25pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for SPINT1 elisa kit

Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.

Product Categories/Family for SPINT1 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 74027265
• NCBI GeneID: 6692
• NCBI Accession #: NP_001027539.1
• NCBI GenBank Nucleotide #: NM_001032367.1
• UniProt Accession #: O43278; Q7Z7D2
• Molecular Weight: 56,885 Da
• NCBI Official Full Name: kunitz-type protease inhibitor 1 isoform 2
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kunitz type 1
• NCBI Official Symbol: SPINT1
• NCBI Official Synonym Symbols: HAI; HAI1; MANSC2
• NCBI Protein Information: kunitz-type protease inhibitor 1
• UniProt Protein Name: Kunitz-type protease inhibitor 1
• Protein Family: Kunitz-type protease inhibitor
• UniProt Gene Name: SPINT1
• UniProt Synonym Gene Names: HAI1; HAI-1
• UniProt Entry Name: SPIT1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

SPINT1: Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: extracellular region; extracellular space; membrane