Mouse Crossveinless-2/CV-2/BMPER ELISA Kit | Bmper elisa kit

Mouse Crossveinless-2/CV-2/BMPER ELISA Kit

Cat. Num. AAA1753897

• Gene Names: Bmper; Cv2; CV-2; Crim3; 3110056H04Rik
• Reactivity: Mouse
• Synonyms: Crossveinless-2/CV-2/BMPER; Mouse Crossveinless-2/CV-2/BMPER ELISA Kit; CV2; CV-2; CRIM3; BMP-binding endothelial regulator; Bmper elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse BMPER

• Samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 62.5pg/ml-4,000pg/ml
• Sensitivity: <50pg/ml
• Capture/Detection Antibodies: Capture antibody- a polyclonal antibody from goat|Detection antibody- a biotinylated polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.059|0.143|0.193|0.307|0.562|0.917|1.452|2.198
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|207|813|1459|; Standard deviation|11.18|46.34|97.75|; CV(%)|5.4%|5.7%|6.7%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|212|846|1558; Standard deviation|12.30|62.60|123.08; CV(%)|5.8%|7.4%|7.9%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 207|220|215|195|209|7.94|3.8%; Sample 2: 813|827|854|792|822|46.85|5.7%; Sample 3: 1459|1322|1478|1334|1398|86.68|6.2%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: V34-R685
• Anticoagulant: heparin, EDTA or citrate
• Principle: Mouse BMPER ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for BMPER has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: V34-R685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMPER is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMPER amount of sample captured in plate.
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml mouse BMPER standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma (heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" for details. We recommended that each mouse BMPER standard solution and each sample be measured in duplicate.
• TMB Incubation Time: 15-20
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for Bmper elisa kit

This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis.

Product Categories/Family for Bmper elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 24371216
• NCBI GeneID: 73230
• NCBI Accession #: NP_082748.1
• NCBI GenBank Nucleotide #: NM_028472.2
• UniProt Accession #: Q8CJ69; Q7TN57; Q80UZ1; Q9CXM8
• Molecular Weight: 76,148 Da
• NCBI Official Full Name: BMP-binding endothelial regulator protein
• NCBI Official Synonym Full Names: BMP-binding endothelial regulator
• NCBI Official Symbol: Bmper
• NCBI Official Synonym Symbols: Cv2; CV-2; Crim3; 3110056H04Rik
• NCBI Protein Information: BMP-binding endothelial regulator protein
• UniProt Protein Name: BMP-binding endothelial regulator protein
• Protein Family: BMP-binding endothelial regulator protein
• UniProt Gene Name: Bmper
• UniProt Synonym Gene Names: Cv2; mCV2

NCBI Description

This gene encodes a secreted protein that contains five Von Willebrand factor type C domains and a Von Willebrand factor type D domain and a trypsin inhibitory-like domain. The encoded protein binds to bone morphogenetic proteins (BMP) and regulates their activity. Mutation of the related gene in humans causes diaphanospondylodysostosis. [provided by RefSeq, Mar 2013]

Uniprot Description

BMPER: Inhibitor of bone morphogenetic protein (BMP) function, it may regulate BMP responsiveness of osteoblasts and chondrocytes. Defects in BMPER are the cause of diaphanospondylodysostosis (DSD). A rare, recessively inherited, perinatal lethal skeletal disorder. The primary skeletal characteristics of the phenotype include a small chest, abnormal vertebral segmentation, and posterior rib gaps containing incompletely differentiated mesenchymal tissue. Consistent craniofacial features include ocular hypertelorism, epicanthal folds, a depressed nasal bridge with a short nose, and low-set ears. The most commonly described extraskeletal finding is nephroblastomatosis with cystic kidneys, but other visceral findings have been described in some cases.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9|9 A3

Cellular Component: extracellular region; extracellular space

Molecular Function: protein binding

Biological Process: blood vessel endothelial cell proliferation involved in sprouting angiogenesis; endothelial cell activation; endothelial cell differentiation; inner ear development; negative regulation of BMP signaling pathway; ureteric bud development