Rabbit anti-Mouse, Rat CD146/Mcam Polyclonal Antibody | anti-Mcam antibody

Anti-CD146/Mcam Antibody

Cat. Num. AAA1752998

• Gene Names: Mcam; CD146; CD149; Muc18; s-endo; AV025631; 1-gicerin; s-gicerin
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: CD146/Mcam; Polyclonal Antibody; Anti-CD146/Mcam Antibody; Cell surface glycoprotein MUC18; Gicerin; Melanoma cell adhesion molecule; Melanoma-associated antigen MUC18; CD146; Mcam; Muc18; anti-Mcam antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 648

• Applicable Applications for anti-Mcam antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.1-0.25ug/ml (Mouse, Rat); IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat); IF: 2ug/ml (Mouse); FC/FACS: 1-3ug/1x106 cells (Mouse, Rat); Direct ELISA: 0.1-0.5ug/ml (Mouse); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived mouse CD146/Mcam recombinant protein (Position: E27-E621).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-Mcam antibody

Description: Rabbit IgG polyclonal antibody for CD146/Mcam detection. Tested with WB, IHC-P, IF, FCM, Direct ELISA in Mouse; Rat.

Background: In humans, the CD146 protein is encoded by the MCAM gene. It is mapped to 9; 9 A5.1. CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM functions as a receptor for laminin alpha 4, a matrix molecule that is broadly expressed within the vascular wall. Accordingly, MCAM is highly expressed by cells that are components of the blood vessel wall, including vascular endothelial cells, smooth muscle cells and pericytes. Its function is still poorly understood, but evidence points to it being part of the endothelial junction associated with the actin cytoskeleton. A member of the Immunoglobulin superfamily, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.

References

1. Bardin, N., Anfosso, F., Masse, J.-M., Cramer, E., Sabatier, F., Le Bivic, A., Sampol, J., Dignat-George, F. Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion. Blood 98: 3677-3684, 2001.
2. Kuske, M. D. A., Johnson, J. P. Assignment of the human melanoma cell adhesion molecule gene (MCAM) to chromosome 11 band q23.3 by radiation hybrid mapping. Cytogenet. Cell Genet. 87: 258 only, 1999.
3. Sers, C., Kirsch, K., Rothbacher, U., Riethmuller, G., Johnson, J. P. Genomic organization of the melanoma-associated glycoprotein MUC18: implications for the evolution of the immunoglobulin domains. Proc. Nat. Acad. Sci. 90: 8514-8518, 1993.

NCBI and Uniprot Product Information

• NCBI GI #: 160333901
• NCBI GeneID: 84004
• NCBI Accession #: NP_075548.2
• NCBI GenBank Nucleotide #: NP_075548.2
• UniProt Accession #: Q8R2Y2
• NCBI Official Full Name: cell surface glycoprotein MUC18 isoform 1
• NCBI Official Synonym Full Names: melanoma cell adhesion molecule
• NCBI Official Symbol: Mcam
• NCBI Official Synonym Symbols: CD146; CD149; Muc18; s-endo; AV025631; 1-gicerin; s-gicerin
• NCBI Protein Information: cell surface glycoprotein MUC18
• UniProt Protein Name: Cell surface glycoprotein MUC18
• Protein Family: Cell surface glycoprotein
• UniProt Gene Name: Mcam
• UniProt Synonym Gene Names: Muc18

Uniprot Description

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration ().