Western Blot (WB)
(Anti- CD146 antibody, Western blottingAll lanes: Anti CD146 at 0.5ug/mlWB: Recombinant Human CD146 Protein 0.5ngPredicted bind size: 48KDObserved bind size: 48KD)
anti-Human CD146 Polyclonal Antibody | anti-CD146 antibody
Anti-CD146 Antibody
Gene Names
MCAM; CD146; MUC18
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Synonyms
CD146; Polyclonal Antibody; Anti-CD146 Antibody; Cell surface glycoprotein MUC18; A32 antigen; CD 146; CD146 antigen; Cell surface glycoprotein P1H12; Gicerin; MCAM; Melanoma adhesion molecule; Melanoma associated antigen A32; Melanoma associated antigen MUC18; Melanoma associated glycoprotein MUC18; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MelCAM; MUC 18; MUC18; MUC18_HUMAN; S endo 1; S endo 1 endothelial associated antigen; S-endo 1 endothelial-associated antigen; Sendo 1 endothelial associated antigen; Sendo1; melanoma cell adhesion molecule; anti-CD146 antibody
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
646
Applicable Applications for anti-CD146 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
Immunogen
E Coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti- CD146 antibody, Western blottingAll lanes: Anti CD146 at 0.5ug/mlWB: Recombinant Human CD146 Protein 0.5ngPredicted bind size: 48KDObserved bind size: 48KD)
Western Blot (WB)
(Anti- CD146 antibody, Western blottingAll lanes: Anti CD146 at 0.5ug/mlWB: Recombinant Human CD146 Protein 0.5ngPredicted bind size: 48KDObserved bind size: 48KD)
Western Blot (WB)
(Anti- CD146 antibody, Western blottingAll lanes: Anti CD146 at 0.5ug/mlLane 1: A375 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 72KDObserved bind size: 100KD)
Western Blot (WB)
(Anti- CD146 antibody, Western blottingAll lanes: Anti CD146 at 0.5ug/mlLane 1: A375 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 72KDObserved bind size: 100KD)
Immunohistochemistry (IHC)
(Anti- CD146 antibody, IHC(P)IHC(P): Human Lung Cancer Tissue)
Immunohistochemistry (IHC)
(Anti- CD146 antibody, IHC(P)IHC(P): Human Lung Cancer Tissue)
Related Product Information for anti-CD146 antibody
Description: Rabbit IgG polyclonal antibody for Cell surface glycoprotein MUC18(MCAM) detection. Tested with WB, IHC-P in Human.
Background: CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.
Background: CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.
References
1. Bardin, N., Anfosso, F., Masse, J.-M., Cramer, E., Sabatier, F., Le Bivic, A., Sampol, J., Dignat-George, F.Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion.Blood 98: 3677-3684, 2001. 2. Kuske, M. D. A., Johnson, J. P.Assignment of the human melanoma cell adhesion molecule gene (MCAM) to chromosome 11 band q23.3 by radiation hybrid mapping.Cytogenet. Cell Genet. 87: 258 only, 1999. 3. Taira, E., Nagino, T., Taniura, H., Takaha, N., Kim, C.-H., Kuo, C.-H., Li, B.-S., Higuchi, H., Miki, N.Expression and functional analysis of a novel isoform of gicerin, an immunoglobulin superfamily cell adhesion molecule.J. Biol. Chem. 270: 28681-28687, 1995.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,605 Da
NCBI Official Full Name
cell surface glycoprotein MUC18
NCBI Official Synonym Full Names
melanoma cell adhesion molecule
NCBI Official Symbol
MCAM
NCBI Official Synonym Symbols
CD146; MUC18
NCBI Protein Information
cell surface glycoprotein MUC18
UniProt Protein Name
Cell surface glycoprotein MUC18
UniProt Gene Name
MCAM
UniProt Synonym Gene Names
MUC18
UniProt Entry Name
MUC18_HUMAN
Uniprot Description
MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: external side of plasma membrane; extracellular space; focal adhesion; integral to membrane; nucleus; plasma membrane
Biological Process: anatomical structure morphogenesis; angiogenesis; cell adhesion; glomerular filtration; positive regulation of cell migration
Research Articles on CD146
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Product Notes
The CD146 mcam (Catalog #AAA178364) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD146 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD146 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot Concentration: 0.1-0.5ug/ml Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml. Researchers should empirically determine the suitability of the CD146 mcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD146, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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