Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse MCAM(CD146) expression on BABL/c splenocytes. Cells were stained with FITC-conjugated anti-Mouse MCAM(CD146) and PE conjugated anti-mouse CD49b (BD Pharmingen'). The dot plots were derived from events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Mouse CD146/MCAM Monoclonal Antibody | anti-MCAM antibody
Recombinant Anti-CD146/MCAM Antibody (FITC), Rabbit Monoclonal
Gene Names
Mcam; CD146; CD149; Muc18; s-endo; AV025631; 1-gicerin; s-gicerin
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD146/MCAM; Monoclonal Antibody; Recombinant Anti-CD146/MCAM Antibody (FITC); Rabbit Monoclonal; CD146/MCAM Antibody (FITC); Rabbit MAb; melanoma cell adhesion molecule; Anti-1-gicerin Antibody; Anti-AV025631 Antibody; Anti-CD146 Antibody; Anti-CD149 Antibody; Anti-Muc18 Antibody; Anti-s-endo Antibody; Anti-s-gicerin Antibody; anti-MCAM antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
241
Specificity
Mouse CD146/MCAM
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.03%ProClin300
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-MCAM antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse CD146/MCAM Protein
Conjugation
FITC
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD146/MCAM (rM CD146/MCAM) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse MCAM(CD146) expression on BABL/c splenocytes. Cells were stained with FITC-conjugated anti-Mouse MCAM(CD146) and PE conjugated anti-mouse CD49b (BD Pharmingen'). The dot plots were derived from events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse MCAM(CD146) expression on BABL/c splenocytes. Cells were stained with FITC-conjugated anti-Mouse MCAM(CD146) and PE conjugated anti-mouse CD49b (BD Pharmingen'). The dot plots were derived from events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-MCAM antibody
The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region, and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumors, and thus has been suggested to play an important role in tumor progression and the development of metastasis in certain human carcinomas.
References
Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,901 Da
NCBI Official Full Name
cell surface glycoprotein MUC18
NCBI Official Synonym Full Names
melanoma cell adhesion molecule
NCBI Official Symbol
Mcam
NCBI Official Synonym Symbols
CD146; CD149; Muc18; s-endo; AV025631; 1-gicerin; s-gicerin
NCBI Protein Information
cell surface glycoprotein MUC18
UniProt Protein Name
Cell surface glycoprotein MUC18
Protein Family
UniProt Gene Name
Mcam
UniProt Synonym Gene Names
Muc18
Uniprot Description
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration ().
Research Articles on MCAM
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Product Notes
The MCAM mcam (Catalog #AAA8112074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD146/MCAM Antibody (FITC), Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD146/MCAM can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the MCAM mcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD146/MCAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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