Typical Testing Data/Standard Curve (for reference only)
General 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit | DHVD3 elisa kit
General 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit
Gene Names
CYP24A1; CP24; HCAI; CYP24; HCINF1; P450-CC24
Reactivity
General
Synonyms
1; 25-Dihydroxyvitamin D3 (DHVD3); General 1; 25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit; DH-VD3; Calcitriol; Rocaltrol; Calcijex; 25-(OH)2D3; 25-Dihydroxycholecalciferol; 25-Dihydroxy Vitamin D3; DHVD3 elisa kit
Reactivity
General
Specificity
This assay has high sensitivity and excellent specificity for detection of DHT. No significant cross-reactivity or interference between DHT and analogues was observed.
Sequence Length
513
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids
Assay Type
Quantitative Competitive
Detection Range
31.2-2000pg/mL
Sensitivity
16.15pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DHT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DHT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DHVD3 elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of DHT in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to DHT has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled DHT and unlabeled DHT (Standards or samples) with the pre-coated antibody specific to DHT. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of DHT in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of DHT in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to DHT has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled DHT and unlabeled DHT (Standards or samples) with the pre-coated antibody specific to DHT. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of DHT in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of DHT in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
43,051 Da
NCBI Official Full Name
1,25-dihydroxyvitamin D3 24-hydroxylase
NCBI Official Synonym Full Names
cytochrome P450 family 24 subfamily A member 1
NCBI Official Symbol
CYP24A1
NCBI Official Synonym Symbols
CP24; HCAI; CYP24; HCINF1; P450-CC24
NCBI Protein Information
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
UniProt Protein Name
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
UniProt Gene Name
CYP24A1
UniProt Synonym Gene Names
CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D3-26,23-lactone as end product.
Research Articles on DHVD3
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Product Notes
The General DHVD3 cyp24a1 (Catalog #AAA2700158) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2700158 ELISA Kit recognizes General DHVD3. It is sometimes possible for the material contained within the vial of "1,25-Dihydroxyvitamin D3 (DHVD3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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