Human MAG ELISA Kit | MAG elisa kit

Human MAG PicoKine ELISA Kit

Cat. Num. AAA1752666

• Gene Names: MAG; GMA; S-MAG; SPG75; SIGLEC4A; SIGLEC-4A
• Reactivity: Human
• Synonyms: MAG; Human MAG PicoKine ELISA Kit; Human MAG; myelin associated glycoprotein; Myelin-associated glycoprotein; Siglec-4a; GMA; MAG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human MAG
• Sequence Length: 626

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 312pg/ml-20000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAG elisa kit

Principle of the Assay: The Picokine™ Human MAG Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MAG with a 96-well strip plate that is pre-coated with antibody specific for MAG. The detection antibody is a biotinylated antibody specific for MAG. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human MAG with immunogen: Expression system for standard: NSO, Immunogen sequence: G20-P516. The kit is analytically validated with ready to use reagents. To measure Human MAG, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MAG in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MAG in the sample.!!Background/Introduction: Myelin-associated glycoprotein (MAG, Siglec-4) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NoGo-66 receptor(NgR). This gene is mapped to 19q13.12. The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 11225258
• NCBI GeneID: 4099
• NCBI Accession #: NP_002352.1
• NCBI GenBank Nucleotide #: NP_002352.1
• UniProt Accession #: P20916
• NCBI Official Full Name: myelin-associated glycoprotein isoform a
• NCBI Official Synonym Full Names: myelin associated glycoprotein
• NCBI Official Symbol: MAG
• NCBI Official Synonym Symbols: GMA; S-MAG; SPG75; SIGLEC4A; SIGLEC-4A
• NCBI Protein Information: myelin-associated glycoprotein
• UniProt Protein Name: Myelin-associated glycoprotein
• Protein Family: Magainins
• UniProt Gene Name: MAG
• UniProt Synonym Gene Names: GMA
• UniProt Entry Name: MAG_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

MAG: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3- linked sialic acid. Binds to RTN4R. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: paranode region of axon; integral to membrane; plasma membrane

Molecular Function: carbohydrate binding

Biological Process: substantia nigra development; nerve growth factor receptor signaling pathway; negative regulation of axonogenesis; regulation of axonogenesis; blood coagulation; cell adhesion; leukocyte migration