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Colloidal Gold Secondary Antibody

10 nm Colloidal Gold Conjugated Protein G

Applications
Immunohistochemistry, Immunocytochemistry
Synonyms
Colloidal Gold; 10 nm Colloidal Gold Conjugated Protein G; Colloidal Gold secondary antibody
Ordering
For Research Use Only!
Form/Format
Concentrated, Liquid
Applicable Applications for Colloidal Gold secondary antibody
Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Electron Microscopy (EM)
Application Notes
EM: 1:20-50
IHC-P: 1:50-100
IHC-F: 1:50-100
ICC: 1:50-100
Relevant Detection Systems
Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing binding specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fab and Fc region. The native molecule also binds albumin, but because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G.
Protein G is conjugated to 10 nm colloidal gold. This gold conjugated protein G generates electron dense particles that are visible using electron microscopy.
Protein G is conjugated to 5 nm colloidal gold. This gold conjugated protein G generates electron dense particles that are visible using electron microscopy.
Preparation and Storage
Store at 4 degree C for one year.

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Product Notes

The Colloidal Gold (Catalog #AAA1751888) is a Secondary Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Colloidal Gold can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Electron Microscopy (EM). EM: 1:20-50 IHC-P: 1:50-100 IHC-F: 1:50-100 ICC: 1:50-100. Researchers should empirically determine the suitability of the Colloidal Gold for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Colloidal Gold, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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