Western Blot (WB)
(Anti-Haptoglobin antibody, MBS175124, Western blottingLane 1:RAJI Cell LysateLane 2:HL-60 Cell LysateLane 3:HUT102 Cell LysateLane 4:JURKAT Cell LysateLane 5:CEM Cell Lysate )
Rabbit anti-Human Haptoglobin Polyclonal Antibody | anti-HP antibody
Anti-Haptoglobin antibody
IHC-P: Concentration: 0.5-1ug/ml Tested Species: Human Antigen Retrieval: By heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Western Blot (WB)
(Anti-Haptoglobin antibody, MBS175124, Western blottingLane 1:RAJI Cell LysateLane 2:HL-60 Cell LysateLane 3:HUT102 Cell LysateLane 4:JURKAT Cell LysateLane 5:CEM Cell Lysate )
Western Blot (WB)
(Anti-Haptoglobin antibody, MBS175124, Western blottingLane 1:RAJI Cell LysateLane 2:HL-60 Cell LysateLane 3:HUT102 Cell LysateLane 4:JURKAT Cell LysateLane 5:CEM Cell Lysate )
Immunohistochemistry (IHC)
(Anti-Haptoglobin antibody, MBS175124, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-Haptoglobin antibody, MBS175124, IHC(P)IHC(P): Human Lung Cancer Tissue )
Background: Haptoglobin(HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. In the chimpanzee, there are 3 genes in the haptoglobin family(haptoglobin, HP; haptoglobin-related, HPR; and haptoglobin-primate, HPP), whereas only 2 genes exist in humans(HP and HPR). There are similarities between the primary structures of the alpha chain and of light chains of gamma globulins. The alpha haptoglobin locus is on the long arm of chromosome 16, where its exact cytogenetic location is 16q22.2. A major function of haptoglobin is to bind hemoglobin(Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. The human haptoglobin HP*2 allele contains a 1.7-kb intragenic duplication that arose after a unique nonhomologous recombination between the prototype HP*1 alleles.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]
Uniprot Description
HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 16q22.2
Cellular Component: extracellular space; extracellular region
Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity
Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; immune system process; metabolic process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response
Disease: Anhaptoglobinemia
Research Articles on HP
Similar Products
Product Notes
The HP hp (Catalog #AAA175124) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Haptoglobin antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Haptoglobin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: Concentration: 0.1-0.5ug/ml Tested Species: Human IHC-P: Concentration: 0.5-1ug/ml Tested Species: Human Antigen Retrieval: By heat Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the HP hp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Haptoglobin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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