Western Blot (WB)
((0.1ug/ml) staining of Human Cerebellum ysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TRABID/ZRANB1 Polyclonal Antibody | anti-ZRANB1 antibody
Goat anti-TRABID/ZRANB1 Antibody
Gene Names
ZRANB1; TRABID
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TRABID/ZRANB1; Polyclonal Antibody; Goat anti-TRABID/ZRANB1 Antibody; ZRANB1; TRABID; zinc finger; RAN-binding domain containing 1; DKFZp762P2216; TRAF-binding protein domain; RAN-binding domain containing 1 protein; ZRANB1 antibody; TRABID antibody; RAN-binding domain containing 1 antibody; DKFZp762P2216 antibody; TRAF-binding protein domain antibody; RAN-binding domain containing 1 protein antibody; TRABID / ZRANB1; anti-ZRANB1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KWLDRYRQIRPCTS
Sequence Length
708
Applicable Applications for anti-ZRANB1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx.80kDa bands observed in Human Brain (Cerebellum) lysates (calculated MW of 81. 0kDa according to NP_060050.2). Recommended concentration: 1-3ug/ml.
Western Blot: Approx.80kDa bands observed in Human Brain (Cerebellum) lysates (calculated MW of 81. 0kDa according to NP_060050.2). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence KWLDRYRQIRPCTS, from the C Terminus of the protein sequence according to NP_060050.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Human Cerebellum ysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Cerebellum ysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,967 Da
NCBI Official Full Name
ubiquitin thioesterase ZRANB1
NCBI Official Synonym Full Names
zinc finger RANBP2-type containing 1
NCBI Official Symbol
ZRANB1
NCBI Official Synonym Symbols
TRABID
NCBI Protein Information
ubiquitin thioesterase ZRANB1
UniProt Protein Name
Ubiquitin thioesterase ZRANB1
Protein Family
UniProt Gene Name
ZRANB1
UniProt Synonym Gene Names
TRABID; hTrabid
UniProt Entry Name
ZRAN1_HUMAN
Uniprot Description
ZRANB1: Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'- linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling. Belongs to the peptidase C64 family.
Protein type: EC 3.4.19.12; Ligase; Protease; Ubiquitin-specific protease; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 10q26.13
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: protein binding; ubiquitin-specific protease activity; zinc ion binding
Biological Process: cell migration; cytoskeleton organization and biogenesis; positive regulation of Wnt receptor signaling pathway; regulation of cell morphogenesis; Wnt receptor signaling pathway
Research Articles on ZRANB1
Similar Products
Product Notes
The ZRANB1 zranb1 (Catalog #AAA421923) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TRABID/ZRANB1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's TRABID/ZRANB1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx.80kDa bands observed in Human Brain (Cerebellum) lysates (calculated MW of 81. 0kDa according to NP_060050.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the ZRANB1 zranb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KWLDRYRQIR PCTS. It is sometimes possible for the material contained within the vial of "TRABID/ZRANB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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