Rabbit MED12 Polyclonal Antibody | anti-MED12 antibody

MED12 Polyclonal Antibody

Cat. Num. AAA3007206

• Gene Names: MED12; Kto; OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MED12; Polyclonal Antibody; MED12 Polyclonal Antibody; Mediator of RNA polymerase II transcription subunit 12; ARC240; CAG repeat protein 45; Mediator complex subunit 12; OPA-containing protein; Trap230; Trinucleotide repeat-containing gene 11 protein; anti-MED12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MED12 polyclonal antibody detects endogenous levels of MED12 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 2177

• Applicable Applications for anti-MED12 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MED12.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MED12 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:H9C2 whole cell lysateLane3:sp20 whole cell lysateLane4:PC12 whole cell lysate)

Related Product Information for anti-MED12 antibody

In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, that are characterized by the presence of a nuclear receptor recognition motif which mediates the ligand-dependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes.

NCBI and Uniprot Product Information

• NCBI GI #: 110347429
• NCBI GeneID: 9968
• NCBI Accession #: NP_005111.2
• NCBI GenBank Nucleotide #: NP_005111.2
• UniProt Accession #: Q93074
• Molecular Weight: ~ 243kDa
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 12
• NCBI Official Synonym Full Names: mediator complex subunit 12
• NCBI Official Symbol: MED12
• NCBI Official Synonym Symbols: Kto; OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 12
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 12
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED12
• UniProt Synonym Gene Names: ARC240; CAGH45; HOPA; KIAA0192; TNRC11; TRAP230; ARC240; Trap230
• UniProt Entry Name: MED12_HUMAN

NCBI Description

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]

Uniprot Description

MED12: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3. Ubiquitous. Belongs to the Mediator complex subunit 12 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: nucleoplasm; centrosome; membrane; nucleolus; Srb-mediator complex; nucleus

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription coactivator activity; beta-catenin binding; transcription cofactor activity; thyroid hormone receptor binding; chromatin binding; receptor activity

Biological Process: steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Schwann cell development; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of transcription, DNA-dependent; heart development; stem cell maintenance; Wnt receptor signaling pathway through beta-catenin; androgen receptor signaling pathway; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; gene expression; oligodendrocyte development

Disease: Ohdo Syndrome, X-linked; Opitz-kaveggia Syndrome; Lujan-fryns Syndrome