Mouse gamma-Tubulin Monoclonal Antibody | anti-GGA1 antibody

Anti-gamma-Tubulin antibody (monoclonal)

Cat. Num. AAA175082

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: Ascites
• Synonyms: gamma-Tubulin; Monoclonal Antibody; Anti-gamma-Tubulin antibody (monoclonal); ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3; golgi-associated; gamma adaptin ear containing; ARF binding protein 1; ARF binding protein 2; ARF binding protein 3; VEAR; KIAA1080; KIAA0154; Gamma-adaptin-related protein 1; Gamma-adaptin-related protein 2; Golgi-localized; gamma ear-containing; ARF-binding protein 2; VHS domain and ear domain of gamma-adaptin; anti-GGA1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: IMD-20
• Purity/Purification: Ascites
• Form/Format: Lyophilized
• Sequence Length: 639

• Applicable Applications for anti-GGA1 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen: Synthetic gamma-tubulin peptide (amino acids 38-53), conjugated to KLH.
• Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
• Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-GGA1 antibody

Description: Mouse IgG monoclonal antibody for gamma-Tubulin, golgi-associated, gamma adaptin ear containing, ARF binding protein 1, golgi-associated, gamma adaptin ear containing, ARF binding protein 2, golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA1, GGA2, GGA3) detection. Tested with WB in Human,rat. No cross reactivity with other proteins. Uniprot ID: Q9UJY5
Background: Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.

NCBI and Uniprot Product Information

• NCBI GI #: 14548066
• NCBI GeneID: 26088
• UniProt Accession #: Q9UJY5; Q5TG07; Q86YA9; Q8NCS6; Q9BW94; Q9UG00; Q9UGW0; Q9UGW1; A8K3D3; B0QYR7
• Molecular Weight: 9,969 Da
• NCBI Official Full Name: ADP-ribosylation factor-binding protein GGA1
• NCBI Official Synonym Full Names: golgi-associated, gamma adaptin ear containing, ARF binding protein 1
• NCBI Official Symbol: GGA1
• NCBI Protein Information: ADP-ribosylation factor-binding protein GGA1; gamma-adaptin related protein 1; gamma-adaptin-related protein 1; ADP-ribosylation factor binding protein 1; golgi-localized, gamma ear-containing, ARF-binding protein 1; golgi associated, gamma adaptin ear containing, ARF binding protein 1
• UniProt Protein Name: ADP-ribosylation factor-binding protein GGA1
• Protein Family: Gamma-tubulin complex
• UniProt Gene Name: GGA1
• UniProt Entry Name: GGA1_HUMAN

NCBI Description

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GGA1: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Belongs to the GGA protein family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; Golgi apparatus; clathrin adaptor complex; intracellular membrane-bound organelle; membrane; endosome membrane

Molecular Function: protein binding

Biological Process: vesicle-mediated transport; intracellular protein transport; positive regulation of protein catabolic process