Western Blot (WB)
(GGA1 rabbit polyclonal antibody. Western Blot analysis of GGA1 expression in K-562.)
Rabbit anti-Human GGA1 Polyclonal Antibody | anti-GGA1 antibody
GGA1 (ADP-ribosylation Factor-binding Protein GGA1, Gamma-adaptin-related Protein 1, Golgi-localized, gamma Ear-containing, ARF-binding Protein 1) (HRP)
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GGA1; Polyclonal Antibody; GGA1 (ADP-ribosylation Factor-binding Protein GGA1; Gamma-adaptin-related Protein 1; Golgi-localized; gamma Ear-containing; ARF-binding Protein 1) (HRP); anti-GGA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GGA1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-GGA1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human GGA1, aa1-552 (NP_001001560.1).
Immunogen Sequence
MEPAMEPETLEARINRATNPLNKELDWASINGFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVLETCMKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTSEKVKNKILELLYSWTVGLPEEVKIAEAYQMLKKQGIVKSDPKLPDDTTFPLPPPRPKNVIFEDEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEKISKRVNAIEEVNNNVKLLTEMVMSHSQGGAAAGSSEDLMKELYQRCERMRPTLFRLASDTEDNDEALGLSDPTPPSGPSLDGTGWNSFQSSDATEPPAPALAQAPSMESRPPAQTSLPASSGLDDLDLLGKTLLQQSLPPESQQVRWEKQQPTPRLTLRDLQNKSSSCSSPSSSATSLLHTVSPEPPRPPQQPVPTELSLASITVPLESIKPSNILPVTVYDQHGFRILFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVFQSAVPKVMKVKLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLTFTMGDQTYNEMGDVDQFPPPETWGSL
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(GGA1 rabbit polyclonal antibody. Western Blot analysis of GGA1 expression in K-562.)
Western Blot (WB)
(GGA1 rabbit polyclonal antibody. Western Blot analysis of GGA1 expression in K-562.)
Western Blot (WB)
(Western Blot analysis of GGA1 expression in transfected 293T cell line by GGA1 polyclonal antibody. Lane 1: GGA1 transfected lysate (61.4kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of GGA1 expression in transfected 293T cell line by GGA1 polyclonal antibody. Lane 1: GGA1 transfected lysate (61.4kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-GGA1 antibody
GGA1 is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.
Product Categories/Family for anti-GGA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9,969 Da
NCBI Official Full Name
ADP-ribosylation factor-binding protein GGA1 isoform 2
NCBI Official Synonym Full Names
golgi-associated, gamma adaptin ear containing, ARF binding protein 1
NCBI Official Symbol
GGA1
NCBI Protein Information
ADP-ribosylation factor-binding protein GGA1; gamma-adaptin related protein 1; gamma-adaptin-related protein 1; ADP-ribosylation factor binding protein 1; golgi-localized, gamma ear-containing, ARF-binding protein 1; golgi associated, gamma adaptin ear co
UniProt Protein Name
ADP-ribosylation factor-binding protein GGA1
Protein Family
UniProt Gene Name
GGA1
UniProt Entry Name
GGA1_HUMAN
Uniprot Description
GGA1: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Belongs to the GGA protein family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 22q13.31
Cellular Component: nucleoplasm; Golgi apparatus; clathrin adaptor complex; intracellular membrane-bound organelle; membrane; endosome membrane
Molecular Function: protein binding
Biological Process: vesicle-mediated transport; intracellular protein transport; positive regulation of protein catabolic process
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Product Notes
The GGA1 gga1 (Catalog #AAA6379740) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GGA1 (ADP-ribosylation Factor-binding Protein GGA1, Gamma-adaptin-related Protein 1, Golgi-localized, gamma Ear-containing, ARF-binding Protein 1) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GGA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GGA1 gga1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GGA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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