Pepsinogen II Active Protein | PGC active protein

Pepsinogen II (PGII/PGC), Human Gastric Mucosa

Cat. Num. AAA173574

• Gene Names: PGC; PEPC; PGII
• Applications: Western Blot
• Purity: >= 90% (SDS-PAGE) may contain minor cleavage products.
• Synonyms: Pepsinogen II; Pepsinogen II (PGII/PGC); Human Gastric Mucosa; Pepsinogen C; PG II; PGC; Gastricsin; Pepsinogen II (PGII; PGC) - >=90% (SDS-PAGE) - Lyophilized; PGC active protein

• Host: Human gastric mucosa
• Purity/Purification: >= 90% (SDS-PAGE) may contain minor cleavage products.
• Form/Format: Powder, lyophilized from tris chloride, pH 8.5
• Sequence Length: 315

• Applicable Applications for PGC active protein: Western Blot (WB)
• Appearance: White to off-white powder.
• Solubility: Clear, colorless (1mg/mL 0.02 M Tris-Chloride, pH= 8.0 buffer)
• Protein: 1.4 mg protein /vial (E ^0.1%280nm= 1.43)
• Specific Activity: 2,920 U/mg protein (after activation)
• Unit Definition: One unit will produce a change in A280 of 0.001 per minute at pH 2.0 and 37°C, measured as TCA-soluble products from hemoglobin.
• Identity: Confirmed by Western Blot.
• Handling: Handle as a potentially hazardous substance
• Reconstitution: After reconstitution maintain at or above pH 8.0 to avoid activation.
• Preparation and Storage: Store at -20°C.

Related Product Information for PGC active protein

MyBioSource is the leading producer and supplier of Human Pepsinogen II/C antigen as a stomach gastric cancer marker for medical research, clinical and manufacturing.

Custom preparations, technical support, bulk quantities and aliquoting available.

Human Pepsinogens are aspartic proteases produced in the gastric mucosa and secreted into the gastric lumen that play a major role in the digestion of porteins after activation of acidic pH. Pepsinogen is synthesized as isoymogens and are classified into two groups, I and II.

Product Categories/Family for PGC active protein

Proteins; Antigens; Enzymes; Standards/controls; Native Proteins; Pepsinogen Ii (pgii; Pgc)

NCBI and Uniprot Product Information

• NCBI GI #: 129796
• NCBI GeneID: 5225
• UniProt Accession #: P20142; Q5T3D7; Q5T3D8; B4DVZ3
• Molecular Weight: 40,600
• NCBI Official Full Name: Gastricsin
• NCBI Official Synonym Full Names: progastricsin (pepsinogen C)
• NCBI Official Symbol: PGC
• NCBI Official Synonym Symbols: PEPC; PGII
• NCBI Protein Information: gastricsin; pepsin C; pepsinogen C; preprogastricsin; pepsinogen group II
• UniProt Protein Name: Gastricsin
• Protein Family: Gastricsin
• UniProt Gene Name: PGC
• UniProt Entry Name: PEPC_HUMAN

NCBI Description

This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009]

Uniprot Description

PGC: Hydrolyzes a variety of proteins. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.23.3

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: extracellular space

Molecular Function: aspartic-type endopeptidase activity

Biological Process: digestion; proteolysis