Western Blot (WB)
(Western blot analysis of extracts from C6 cells, using DLST Antibody. The lane on the left was treated with blocking peptide.Observed bands: 40 kDa.)
Rabbit DLST Polyclonal Antibody | anti-DLST antibody
DLST Antibody
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%)
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from C6 cells, using DLST Antibody. The lane on the left was treated with blocking peptide.Observed bands: 40 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from C6 cells, using DLST Antibody. The lane on the left was treated with blocking peptide.Observed bands: 40 kDa.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612430 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612430 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612430 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612430 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612430 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612430 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612430 at 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612430 at 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Subcellular Location: Mitochondrion matrix. Nucleus. Note: Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation.
Subunit Structure: The 2-oxoglutarate dehydrogenase complex is composed of OGDH (2-oxoglutarate dehydrogenase; E1), DLST (dihydrolipoamide succinyltransferase; E2) and DLD (dihydrolipoamide dehydrogenase; E3). It contains multiple copies of the three enzymatic components (E1, E2 and E3). In the nucleus, the 2-oxoglutarate dehydrogenase complex associates with KAT2A (PubMed:29211711).
Similarity: Belongs to the 2-oxoacid dehydrogenase family.
NCBI and Uniprot Product Information
Predicted Molecular Weight: (Calculated)49kDa.
NCBI Description
This gene encodes a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
DLST: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Forms a 24-polypeptide structural core with octahedral symmetry. Belongs to the 2-oxoacid dehydrogenase family.
Protein type: Mitochondrial; Transferase; EC 2.3.1.61; Carbohydrate Metabolism - citrate (TCA) cycle; Amino Acid Metabolism - lysine degradation
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: membrane; mitochondrial matrix; oxoglutarate dehydrogenase complex; nucleus
Molecular Function: dihydrolipoyllysine-residue succinyltransferase activity
Biological Process: cellular metabolic process; generation of precursor metabolites and energy; tricarboxylic acid cycle; L-lysine catabolic process to acetyl-CoA via saccharopine; lysine catabolic process
Research Articles on DLST
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Product Notes
The DLST dlst (Catalog #AAA9612430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DLST Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's DLST can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the DLST dlst for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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