SDS-PAGE
Pepsinogen C / PGC Recombinant Protein | PGC recombinant protein
Recombinant Human Pepsinogen C / PGC Protein (His tag)
Gene Names
PGC; PEPC; PGII
Purity
> 95 % as determined by SDS-PAGE
Synonyms
Pepsinogen C / PGC; Recombinant Human Pepsinogen C / PGC Protein (His tag); PGC; PGC recombinant protein
Host
E Coli
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, 0.4M sucrose, 1mM EDTA, 50mM NaCl, pH 8.0
Sequence Length
315
Application Notes
The recombinant human PGC consists of 102 amino acids and predicts a molecular mass of 10.9 KDa. It migrates as an approximately 10 KDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
His
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for PGC recombinant protein
Background: Pepsinogen C, also known as PGC, is an aspartic proteinase that belongs to the peptidase family A1. Pepsinogen C is synthesized in the gastric mucosa as inactive precursors, known as zymogens. Pepsinogen C contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. At low PH conditions, Pepsinogen C undergoes conversion into active enzyme. Pepsinogen C has been found expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. Serum levels of Pepsinogen C are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.
Description: A DNA sequence encoding the mature form of human PGC (P20142-1) (Ile153-Ile239) was expressed with a polyhistide tag at the N-terminus.
Description: A DNA sequence encoding the mature form of human PGC (P20142-1) (Ile153-Ile239) was expressed with a polyhistide tag at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,208 Da
NCBI Official Full Name
gastricsin isoform 2 preproprotein
NCBI Official Synonym Full Names
progastricsin
NCBI Official Symbol
PGC
NCBI Official Synonym Symbols
PEPC; PGII
NCBI Protein Information
gastricsin
UniProt Protein Name
Gastricsin
Protein Family
UniProt Gene Name
PGC
NCBI Description
This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009]
Uniprot Description
Hydrolyzes a variety of proteins.
Research Articles on PGC
Similar Products
Product Notes
The PGC pgc (Catalog #AAA2545586) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The recombinant human PGC consists of 102 amino acids and predicts a molecular mass of 10.9 KDa. It migrates as an approximately 10 KDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the PGC pgc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pepsinogen C / PGC, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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