cGMP ELISA Kit

cGMP ELISA Kit (Colorimetric)

Cat. Num. AAA168636

• Synonyms: cGMP; cGMP ELISA Kit (Colorimetric); cGMP elisa kit

• Samples: Cell Culture Supernatants, plasma, serum, saliva, urine, and Cell lysates
• Preparation and Storage: Store kit components at 4 degree C until their expiration dates. For longer term use, store the Rabbit Anti-cGMP Polyclonal Antibody at -20 degree C.

Testing Data

Testing Data #2

Testing Data #3

Related Product Information for cGMP elisa kit

Principle of the assay: An anti-Rabbit IgG polyclonal coating antibody is adsorbed onto a microtiter plate. Cyclic GMP present in the sample or standard competes with Peroxidase cGMP Tracer for plate binding, in the presence of Rabbit Anti-cGMP Polyclonal Antibody. Following incubation and wash steps, any Peroxidase cGMP Tracer bound to the plate is detected with addition of Substrate Solution. The colored product formed in inversely proportional to the amount of cGMP present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from cGMP Standard and sample concentration is then determined.

Background: Guanosine 3',5'-cyclic monophosphate (cGMP) is a multi-functional second messenger involved in various cellular activities in many cell and tissue types. It is converted from guanosine triphosphate (GTP) via guanylyl cyclases (GC). It has been shown that the level of cGMP is typically 10-100 fold lower than cAMP in most tissues. cGMP primarily affects cellular activities through four different pathways. These include cGMP-dependent Protein Kinases (PKG/GK), cyclic nucleotide-gated (CNG) channels, cAMP-dependent Protein Kinase (PKA), and Phosphodiesterases. PKGs (PKG I and PKG II) are serine/threonine kinases activated by cGMP. PKG I has several putative targets, many of which are involved in the regulation of smooth muscle cell (SMC) contractility. Other cGMP/PKG I substrates that affect contractility include the myosin-binding subunit (MBS) of MLC Phosphatase and the small GTPase Rho. In addition to its role in smooth muscle relaxation, cGMP/PKG I may also regulate cell survival, proliferation, axon guidance, synaptic plasticity, inflammation, and angiogenesis. cGMP ELISA Kit is a competitive enzyme immunoassay designed to measure cGMP in cell culture supernatants, plasma, serum, saliva, urine, and cell lysates. The kit selectively measures cGMP levels without any significant cross reactivities to other nucleotides or cyclic nucleotides. Samples containing low cGMP levels may be acetylated (reagents provided) for increased sensitivity. Under non-acetylated conditions, the kit has a detection range of 1 to 1000 pmol/mL cGMP; however, under acetylated conditions, the sensitivity is enhanced (approx 50X) to a detection range of 100-6250 fmol/mL.

Product Categories/Family for cGMP elisa kit

Cell Signaling and Protein Biology; G Protein Signaling/Small GTPase Assays and Vectors; Cyclic GMP Assays

References

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