Rabbit anti-Human, Mouse TIM-4 Polyclonal Antibody | anti-TIMD4 antibody

TIM-4 Antibody

Cat. Num. AAA151467

• Gene Names: TIMD4; TIM4; SMUCKLER
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: TIM-4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TIM-4; Polyclonal Antibody; TIM-4 Antibody; TIM4; SMUCKLER; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; TIMD-4; T-cell immunoglobulin and mucin domain containing 4; anti-TIMD4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TIM-4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 378

• Applicable Applications for anti-TIMD4 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: TIM-4 antibody can be used for the detection of TIM-4 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TIM-4 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-4.
• Buffer: TIM-4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TIM-4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunocytochemistry (IHC)

(Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of TIM-4 in Jurkat cells with TIM-4 antibody at 20 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TIM-4 in human spleen tissue with TIM-4 antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of TIM-4 in human spleen tissue with TIM-4 antibody at 20 μg/ml.Green: TIM-4 Antibody (3815)Blue: DAPI staining)

Immunohistochemistry (IHC)

(Immunohistochemistry of TIM-4 in mouse liver tissue with TIM-4 antibody at 2 μg/ml.)

Related Product Information for anti-TIMD4 antibody

TIM-4 Antibody: The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.

NCBI and Uniprot Product Information

• NCBI GI #: 226529863
• NCBI GeneID: 91937
• NCBI Accession #: NP_612388
• NCBI GenBank Nucleotide #: NM_138379.2
• UniProt Accession #: Q96H15; B5MCL9
• Molecular Weight: 38,536 Da
• NCBI Official Full Name: T-cell immunoglobulin and mucin domain-containing protein 4 isoform 1
• NCBI Official Synonym Full Names: T-cell immunoglobulin and mucin domain containing 4
• NCBI Official Symbol: TIMD4
• NCBI Official Synonym Symbols: TIM4; SMUCKLER
• NCBI Protein Information: T-cell immunoglobulin and mucin domain-containing protein 4; TIM-4; TIMD-4; T-cell membrane protein 4; T-cell immunoglobulin mucin receptor 4
• UniProt Protein Name: T-cell immunoglobulin and mucin domain-containing protein 4
• Protein Family: T-cell immunoglobulin and mucin domain-containing protein
• UniProt Gene Name: TIMD4
• UniProt Synonym Gene Names: TIM4; TIMD-4; TIM-4
• UniProt Entry Name: TIMD4_HUMAN

Uniprot Description

TIM-4: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1. Belongs to the immunoglobulin superfamily. TIM family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane